Sequences from confirmation amplicon


Confirmation sequences for line 145G08 and hit At5g20830 are shown.
Line ID 145G08
Locus At5g20830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7051222
Primers used for PCR MR36 - 8474, show primer details
   
Sequence Name 29-K015721-022-145-G08C-8409
GenBank Accession LN499069 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K015721-022-145-G08C-8409
CATGTAGATTTCCCCGACATGAAGCCATTTACCCGGTACAGCTCACCGTTCCTTACCCGG
TCCATCTGAGAGGAGATCCACCTGAACTGACCGTTTAGCTTGTATTCCTCAATGAGATCA
TACATTTTCTTCATCTCTGCTTTCTCTTCATTGTCCTTTGACTCTTTCCTCCTGTCTCCT
CCAACAACAACCAAGTTAGCTAGCTCACGCAAGCGGGTGTTCTTCCCGTACCACTCAACA
AGACCTGACAAGTTCTTGACACGATCAAGCCTAGCCATTGTGAAGAGAATCGGCTTCTTC
TTGTCCTTGAGCACACATCTGTTTCAAAACATCAGATACATTAGAACGACACTCAAAATG
TTAAAGATGAACATGAAGAGTGAACAAAAGCTTACAAGTGCTCTTTGTTCTCAACATCGC
TGTAGAGGAGCTCCTCGATCTCAGAGTGGAACTTAGTCAATCTACGCTTCTCCTCTGTGT
AAGGG
   
Sequence Name 30-K015721-022-145-G08C-MR36
GenBank Accession LN499505 [GenBank]
Sequencing primer gene specific primer, MR36: 5'-GCATCTACTTCCCTTACACAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K015721-022-145-G08C-MR36
ACGTAGATTGACTAAGTTCCACTCTGAGATCGAGGAGCTCCTCTACAGCGATGTTGAGAA
CAAAGAGCACTTGTAAGCTTTTGTTCACTCTTCATGTTCATCTTTAACATTTTGAGTGTC
GTTCTAATGTATCTGATGTTTTGAAACAGATGTGTGCTCAAGGACAAGAAGAAGCCGATT
CTCTTCACAATGGCTAGGCTTGATCGTGTCAAGAACTTGTCAGGTCTTGTTGAGTGGTAC
GGGAAGAACACCCGCTTGCGTGAGCTAGCTAACTTGGTTGTTGTTGGAGGAGACAGGAGG
AAAGAGTCAAAGGACAATGAAGAGAAAGCAGAGATGAAGAAAATGTATGATCTCATTGAG
GAATACAAGCTAAACGGTCAGTTCAGGTGGATCTCCTCTCAGATGGACCGGGTAAGGAAC
GGTGAGCTGTACCGGGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGC

Last Updated on 06.12.2018 16:11