Sequences from confirmation amplicon


Confirmation sequences for line 152A03 and hit At1g33090 are shown.
Line ID 152A03
Locus At1g33090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11995453
Primers used for PCR BM08 - 8474, show primer details
   
Sequence Name 19-K015835-022-152-A03C-8409
GenBank Accession LN494540 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K015835-022-152-A03C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGTGGACATACAAATTAT
AATATTTTACAAATCGACTAATCTTTTCCATTTCTTTTTTGCAGCATCAACGTAAATGCA
CTGCAGATGATGATAGCACTTGGGTTTTTGGCAGCAGTAAGGTGCATTGATATATATACT
ATACACAGATACACTTCACTTCACTGATACTAATTATATCTTATCTTTTTTTTTCTTGGT
ATGATCTTAATATTATTTATATATCACTCTTCTATGTAATTATCTCTATATATAGTGTAA
GAGTGTCCAACGAGCTCGGTAGGGGAAACCCCGAAGGCGCCAAATTTGCAACAATCGTAG
CGGTTTTCACGTCACTCTCTATAGGATTAGTTCTATTCTTCGCCTTCTTGTTTCTAAGGG
GAAGAATTTCGTACATTTTCACTACAAGTGAAGCTGTGGCAGCCGAAGTCGCTGATCTTT
CTCCACTTTTAGCATTT
   
Sequence Name 20-K015835-022-152-A03C-BM08
GenBank Accession LN494990 [GenBank]
Sequencing primer gene specific primer, BM08: 5'-CAAGAGGATGGAAAATGCTAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K015835-022-152-A03C-bm08
GATCACGACTTCGGCTGCCACAGCTTNTACACTTGTAGTGAAAATGTACGAAATTCTTCC
CCTTAGAAACAAGAAGACGAAGAATAGAACTAATCCTATAGAGAGTGACGTGAAAACCGC
TACGATTGTTGCAAATTTGGCGCCTTCGGGGTTTCCCCTACCGAGCTCGTTGGACACTCT
TACACTATATATAGAGATAATTACATAGAAGAGTGATATATAAATAATATTAAGATCATA
CCAAGAAAAAAAAAGATAAGATATAATTAGTATCAGTGAAGTGAAGTGTATCTGTGTATA
GTATATATATCAATGCACCTTACTGCTGCCAAAAACCCAAGTGCTATCATCATCTGCAGT
GCATTTACGTTGATGCTGCAAAAAAGAAATGGAAAAGATTAGTCGATTTGTAAAATATTA
TAATTTGTATGTCCACTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11