Sequences from confirmation amplicon


Confirmation sequences for line 155E07 and hit At3g47570 are shown.
Line ID 155E07
Locus At3g47570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17529298
Primers used for PCR ML61 - 8474, show primer details
   
Sequence Name 08-K100810-0022-155-E07C-8409
GenBank Accession LN489654 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K100810-0022-155-E07C-8409
TCATTGTTAGACAAATCAACTTCTTTAACACCCACCAACCCTTTTAAATCTGGAATATCT
CCATAAAACAAATTTCCTTCCAAAAAAAGACTTTCCATTGTAAGACAGTTTCCCAAAGTT
TGTGGAAGTTTCCCTGATAATTTATTATCCCCAAGTGATAGTGTACCAAGATTTTGTAGC
GCTCCGATATCCTGCGGGAGAGAGCCGATCAAAGAATTTCCTGACATGTCTAGGCGAAGA
AGCTGCTGAATTTTCATAATTTCCAGAGGTATAGTCCCATTCAA
   
Sequence Name 08-K100824-0022-155-E07C-ML61
GenBank Accession LN489656 [GenBank]
Sequencing primer gene specific primer, ML61: 5'-GTTGAATGGGACTATACCTCTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K100824-0022-155-E07C-ML61
GTCAGGGAATTCTTTGATCGGCTCTCTCCCGCAGGATATCGGAGCGCTACAAAATCTTGG
TACACTATCACTTGGGGATAATAAATTATCAGGGAAACTTCCACAAACTTTGGGAAACTG
TCTTACAATGGAAAGTCTTTTTTTGGAAGGAAATTTGTTTTATGGAGATATTCCAGATTT
AAAAGGGTTGGTGGGTGTTAAAGAAGTTGATTTGTCTAACAATGATCTCTCTGGAAGTAT
ACCTGAATACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA

Last Updated on 06.12.2018 16:11