Sequences from confirmation amplicon


Confirmation sequences for line 155G12 and hit At3g21140 are shown.
Line ID 155G12
Locus At3g21140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7410209
Primers used for PCR 26ra - 8474, show primer details
   
Sequence Name 11-K115950-0022-155-G12C-26ra
GenBank Accession LN491269 [GenBank]
Sequencing primer gene specific primer, 26ra: 5'-TAGTGTCTCAACACCATGACCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115950-0022-155-G12C-26ra
TACTGCAAAAACAAACAAACAATTGAACAAGAAAGATAAGCTGAGAAGCACTGCGCATGA
TCATAATTGTGTCGACATCAACTGGACAAAACTAATTAATACAGTGTTATTTTACCTGAG
CACCTTGACGAACCCGTACATCTATTCCTTTGCTATCTATTGAAATGAGAGCAGCATCGT
CTACCTCTGATTCAGTAGACAGTAGCTCTCTCAGCGGTTTCGAGAAGATGGCATTTAGTT
CCTATCAGAAGTTTTAGGGAAAATACGTTAGAGCACACTCGAATCAAATCCAGCTTACAA
AAGGCCAAGAATGAGGAGCAAGTTTATTGGAGTCTCTAAGGCGACCAACAGGGATTTGAA
ACAACGCACCTTGAGGTTCCGCTCACCACCATCAACAGCTACATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCACACAATTATT
   
Sequence Name 01-K115949-0022-155-G12C-8409
GenBank Accession LN486716 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K115949-0022-155-G12C-8409
ACTGTTGATGGTGGTGGCGGAACCTCAGGTGCGTTGTTTCAAATCCCTGTTGGTCGCCTT
AGAGACTCCAATAAACTTGCTCCTCATTCTTGGCCTTTTGTAAGCTGGATTTGATTCGAG
TGTGCTCTAACGTATTTTCCCTAAAACTTCTGATAGGAACTAAATGCCATCTTCTCGAAA
CCGCTGAGAGAGCTACTGTCTACTGAATCAGAGGTAGACGATGCTGCTCTCATTTCAATA
GATAGCAAAGGAATAGATGTACGGGTTCGTCAAGGTGCTCAGGTAAAATAACACTGTATT
AATTAGTTTTGTCCAGTTGATGTCGACACAATTATGATCATGCACAGTGCTTCTCAGCTT
ATCTTTCTTTGTTCAATTGTTTGTTTTGTTTTTTTGCAGTTTAACATACAAAGGCTAGCA
TTTGAGGAAGGTCATGGTGTGNAGACACTAA

Last Updated on 06.12.2018 16:11