Sequences from confirmation amplicon


Confirmation sequences for line 156B03 and hit At3g43300 are shown.
Line ID 156B03
Locus At3g43300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15244721
Primers used for PCR BE53 - 8474, show primer details
   
Sequence Name 81-K028753-022-156-B03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K028753-022-156-B03C-8409
CAAAACGCCTTCGAGTTCAAAAAATCCACCAGCCGCCATTCTTTAA
   
Sequence Name 81-K028753-022-156-B03C-BE53
GenBank Accession LN514007 [GenBank]
Sequencing primer gene specific primer, BE53: 5'-GATTTTGCGGTAGAACAAGAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K028753-022-156-B03C-BE53
CTTAAGGGGTCCGGAGGAAAGAAGTTTCCCGATCTCCAGAAAGCTATTCAAGCTTATCAA
GGTTAGATCTCAATTTCTCCCAATTTTTTGCTCAAAATCGTTCGATCGTTGCTTTTGGTC
CGGGTAGATTCCACGATCATAGCCATCTGCTAGATGTATTTGACTAAAATGTGGTCGCAC
AAGCTGAGATTTCAGGTTATTCCCAGTGTTTGGTTTTGTTAGTGAACTTTAGTATGATGT
GGTAGGATACAGCTAAGACTATGAAATTCGATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11