Sequences from confirmation amplicon


Confirmation sequences for line 156D08 and hit At3g03110 are shown.
Line ID 156D08
Locus At3g03110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:713435
Primers used for PCR TM02 - 8474, show primer details
   
Sequence Name 07-K018911-022-156-D08C-8409
GenBank Accession LN489053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K018911-022-156-D08C-8409
AAATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGACAACTGTCTTCAAAA
ACTTCCAATGGGCTCTTAAGAATCTTGAATATTGTCCAACAACGTACCTGAAGCGAACAA
GAATGCAAAGATCAATACTCACCCGAATCATGCATGAAACAAATGCTAGCAGGGAACAAG
GGGTACTTCTAGTGAAAATCCCACATTACATAAGTACCAACATTCCAAGCCAAAATCAAC
AATAAAATGTGTAGGATCCATGATGTAGAAACAAACTCACATGATGTTGCTCGCAATAAC
AGCTTTATTGTCTTTTCCCTTGACGACTTCACATAAACTTAACAAATCACGGATAACCAT
CACGAGAAATCTGTTTTCCTGGAAAAGAAAGACAGAGTTGAGATGATTAGTAAAGGACAC
AAACTTTGCAAAACACTGGTGGTGAAACCAGTCGACCATAAATCAATGGGAGACCGAGAC
CACTTAAGAGCCTACTAACATTAGAAAGATGCTACCAGATACCAAGAACCAAAAAGTGCT
ACTGCATTTAACTGTTGTTTGTTGAAAACACAATTAGACGGACACTGAGAGTGGAGATCT
TACCTGTTCTA
   
Sequence Name 03-K018911-022-156-D08C-TM02
GenBank Accession LN487284 [GenBank]
Sequencing primer gene specific primer, TM02: 5'-GGGTCCATGGTAGTAGAACAGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K018911-022-156-D08C-TM02
CACTCTCACTGTCCGTCTAATTGTGTTTTCAACAAACAACAGTTAAATGCAGTAGCACTT
TTTGGTTCTTGGTATCTGGTAGCATCTTTCTAATGTTAGTAGGCTCTTAAGTGGTCTCGG
TCTCCCATTGATTTATGGTCGACTGGTTTCACCACCAGTGTTTTGCAAAGTTTGTGTCCT
TTACTAATCATCTCAACTCTGTCTTTCTTTTCCAGGAAAACAGATTTCTCGTGATGGTTA
TCCGTGATTTGTTAAGTTTATGTGAAGTCGTCAAGGGAAAAGACAATAAAGCTGTTATTG
CGAGCAACATCATGTGAGTTTGTTTCTACATCATGGATCCTACACATTTTATTGTTGATT
TTGGCTTGGAATGTTGGTACTTATGTAATGTGGGATTTTCACTAGAAGTACCCCTTGTTC
CCTGCTAGCATTTGTTTCATGCATGATTCGGGTGAGTATTGATCTTTGCATTCTTGTTCG
CTTCAGGTACGTTGTTGGACAATATTCAAGATTCTTAAGAGCCCATTGGAAGTTTTTGAA
GACAGTTGTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCT

Last Updated on 06.12.2018 16:11