Sequences from confirmation amplicon


Confirmation sequences for line 156D11 and hit At3g19670 are shown.
Line ID 156D11
Locus At3g19670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6835601
Primers used for PCR BH69 - 8474, show primer details
   
Sequence Name 12-K104381-0022-156-D11C-BH69
GenBank Accession LN491561 [GenBank]
Sequencing primer gene specific primer, BH69: 5'-TTCTCAGAACTTTCAATGTGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104381-0022-156-D11C-BH69
CCTCAATCTTATGCACCTCAGTTATTACAATCCATGCACCATTCACATGAAAGACCCAGC
CAATTAAATCAGGTGCAAGTGCAACATGTGCCACTTGGACCCCCAACTCTAATTTCACAG
CCAAATGTGTCAATTGCTTCTGGTACCTCCTTGCATCAGCCTTATGTACAAACTCCAGAT
ATTGGTATGCCTGGCTTTGGTGGCCCTAGGGCACTCTTTTCTTATCCGGTAAGGAAGGAA
GATAGTTTTATATTTATATTTACAGAAACTAGACTTCTAGTTCCTAACTCTGTGAAAAAA
CAGAGTGCCACATCTTACGAAGGTTCGAGGGTGCCACCTCAAGTCACTGGACCAAGCATT
CATAGTCAGGCACAACAGAGAGCATCTATCATTCATACTTCTGCAGAATCTTCTATCATG
AATCCGACATTTGAACAACCAAAGATTAATTGAATCAGCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 28-K104382-0022-156-D11C-8409
GenBank Accession LN498828 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104382-0022-156-D11C-8409
GAGCCTTTACAATTGCTGATTCAATTAATCTTTGGTTGTTCAAATGTCGGATTCATGATA
GAAGATTCTGCAGAAGTATGAATGATAGATGCTCTCTGTTGTGCCTGACTATGAATGCTT
GGTCCAGTGACTTGAGGTGGCACCCTCGAACCTTCGTAAGATGTGGCACTCTGTTTTTTC
ACAGAGTTAGGAACTAGAAGTCTAGTTTCTGTAAATATAAATATAAAACTATCTTCCTTC
CTTACCGGATAAGAAAAGAGTGCCCTAGGGCCACCAAAGCCAGGCATACCAATATCTGGA
GTTTGTACATAAGGCTGATGCAAGGAGGTACCAGAAGCAATTGACACATTTGGCTGTGAA
ATTAGAGTTGGGGGTCCAAGTGGCACATGTTGCACTTGCACCTGATTTAATTGGCTGGGT
CTTTCATGTGAATGGTGCATGGATTGTAATAACTGAGGTGCATAAGATTGAGGTGGATAT
CCAATGCTCAATACTGTGCCACCTCTGCCAACACATTGA
Primers used for PCR 04ah - 8474, show primer details
   
Sequence Name 37-K118837-0022-156-D11C-04ah
GenBank Accession LN502795 [GenBank]
Sequencing primer gene specific primer, 04ah: 5'-AAGCAAGAGATTGGTTCATTGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118837-0022-156-D11C-04ah
GACATTGTATCCGAAATCAAGAATCAAAAATATATGACTAAGAAACATTAAAAATAAAAA
ACCTCCTCAGAGAGCACAAAAAATTCCATAAATTTACTTAATAAAGAAACTATCATCTGG
ATTAGCCAATGATATGGTCTCCAAATACCACACAGTGGTAAAAATAGAGAAACAACTACA
CAAACTATCCCGGACATATCTTTTCCAGAATTAGCTTTTGCACAACCTTATTGGGTACTT
CCAAGTTACCAGCAATTTGTCTCGTTTAAATGTCAATATTTGAAATTTGGCATCATATGT
GAAAACGCAAGCAAAACAATCAAAAAAGTATAGATAAAAGTCACAAGACTTATAATTCTT
ACTTCCTTCCATCCGCAGAGGTATGCTCAACCCAATCTGTTAGAGCCTTTTGAGAAGGTA
AAGGTTTTAAAAAAGCTGCCTTCAAGTCGCAAAAGTAAATATAAATTAGTATTCAACCAA
AAAAAGAGTATAGAAGATTCAATACATGCATGCATACAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 13-K118836-0022-156-D11C-8409
GenBank Accession LN492270 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118836-0022-156-D11C-8409
AATTGAATATATCCTGTATGCATGCATGTATTGAATCTTCTATACTCTTTTTTTGGTTGA
ATACTAATTTATATTTACTTTTGCGACTTGAAGGCAGCTTTTTTAAAACCTTTACCTTCT
CAAAAGGCTCTAACAGATTGGGTTGAGCATACCTCTGCGGATGGAAGGAAGTAAGAATTA
TAAGTCTTGTGACTTTTATCTATACTTTTTTGATTGTTTTGCTTGCGTTTTCACATATGA
TGCCAAATTTCAAATATTGACATTTAAACGAGACAAATTGCTGGTAACTTGGAAGTACCC
AATAAGGTTGTGCAAAAGCTAATTCTGGAAAAGATATGTCCGGGATAGTTTGTGTAGTTG
TTTCTCTATTTTTACCACTGTGTGGTATTTGGAGACCATATCATTGGCTAATCCAGATGA
TAGTTTCTTTATTAAGTAAATTTATGGAATTTTTTGTGCTCTCTGAGGAGGTTTTTTATT
TTTAATGTTTCTTAGTCATATATTTTTGATTCTTTGATTTCGGTATACAATTGTCAGCTC
TGTTAAATGTAATGGTATGTGATATGTTCACTCAATGAACCATCTTCTTGCT

Last Updated on 06.12.2018 16:11