Sequences from confirmation amplicon


Confirmation sequences for line 157A04 and hit At5g56970 are shown.
Line ID 157A04
Locus At5g56970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23047768
Primers used for PCR MI09 - 8474, show primer details
   
Sequence Name 25-K018319-022-157-A04C-MI09
GenBank Accession LN497231 [GenBank]
Sequencing primer gene specific primer, MI09: 5'-AACAATAGCATAACCAAAAATGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K018319-022-157-A04C-MI09
AATCTAAATTGCTAAAAGACGAAAAAACTTCGAAAAACGTACGAAGTTCTTTGTTGTCTA
TCAGAAACGTACGAAGTTAAAATCACTATTACTGTTTTTTTTAATCTCTGTGTGAAAAAG
ATCGTGGTACGTACCAGTAATAACATCCATCTCTAGAACATTAGTGATCTGTGGACCGTA
CCGAAACGTTTGTCCACTAATTCCGCCGTTTGATAACGTCCCACCGACTGTTAAATACAA
ATAATCCGTCCAAGAAACCGGCGTTAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTCCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 29-K018319-022-157-A04C-8409
GenBank Accession LN499083 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K018319-022-157-A04C-8409
ATGTAGATTTCCTCGACGATGAAGTCCATTAACGCCGGTTTCTTGGACGGATTATTTGTA
TTTAACAGTCGGTGGGACGTTATCAAACGGCGGAATTAGTGGACAAACGTTTCGGTACGG
TCCACAGATCACTAATGTTCTAGAGATGGATGTTATTACTGGTACGTACCACGATCTTTT
TCACACAGAGATTAAAAAAAACAGTAATAGTGATTTTAACTTCGTACGTTTCTGATAGAC
AACAAAGAACTTCGTACGTTTTTCGAAGTTTTTTCGTCTTTTTCATTTTAGATCTGCGCG
GCCATTTTTGGTTATGCTATTG

Last Updated on 06.12.2018 16:11