Sequences from confirmation amplicon


Confirmation sequences for line 166A05 and hit At1g64150 are shown.
Line ID 166A05
Locus At1g64150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23809671
Primers used for PCR RK34 - 8474, show primer details
   
Sequence Name 61-K015913-022-166-A05C-8409
GenBank Accession LN510386 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K015913-022-166-A05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCATTAGTATCT
CGTTTTTGTATATCCTGCCCGGGGAACTTTTAAAACCATGATTAAAGCTAGTAATTTGCC
ATTAGTATCTCGTTTTTGTGTAATAAAAGCTGAATATATCCTAGGATATGCTGGCATTTA
GTATATTCACCCTAAATTTTCATGATAATCTAAGGACATGGATTTGTTTCATATTTCATT
AATTGTTTTCTACTTTTTTAATGCAACCATTAAAGTTTTATTCTTCTTAACCTTTTACTT
GGTCAGGATGCAGGTTAAGACATGTAACAAAAATCTAAACCTGACACTATAAATGAAAAA
GGAGTATTCTTCAAGTGCTTTTGTGAAATAATGATTTTCTAAAATTGATTTGATCTATAT
AAAGTATAAACATGTATATACTGTCTATGTGTATGTATATAGAGATAGATAAAGAACGGA
ATTAGTTACCTTCTCTGATAAGAAATTTCCTAGCAAAGAACCGCCCAATACTGCAAGCTG
CGATTAACAAACCAAGCTGCAAGTTAGAGTTCTTGTGTCTTCCAAG
   
Sequence Name 62-K015913-022-166-A05C-RK34
GenBank Accession LN510636 [GenBank]
Sequencing primer gene specific primer, RK34: 5'-AATTCTCCTGAAACTTGGAAGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K015913-022-166-A05C-rk34
AAGAACTCTAACTTGCAGCTTGGTTTGTTAATCGCAGCTTGCAGTATTGGGCGGTTCTTT
GCTAGGAAATTTCTTATCAGAGAAGGTAACTAATTCCGTTCTTTATCTATCTCTATATAC
ATACACATAGACAGTATATACATGTTTATACTTTATATAGATCAAATCAATTTTAGAAAA
TCATTATTTCACAAAAGCACTTGAAGAATACTCCTTTTTCATTTATAGTGTCAGGTTTAG
ATTTTTGTTACATGTCTTAACCTGCATCCTGACCAAGTAAAAGGTTAAGAAGAATAAAAC
TTTAATGGTTGCATTAAAAAAGTAGAAAACAATTAATGAAATATGAAACAAATCCATGTC
CTTAGATTATCATGAAAATTTAGGGTGAATATACTAAATGCCAGCATATCCTAGGATATA
TTCAGCTTTTATTACACAAAAACGAGATACTAATGGCAAATTACTAGCTTTAATCATGGT
TTTAAAAGTTCCCCGGGCAGGATATACAAAAACGAGATACTAATGACAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTNTCAATTCAAAAATGTAGATGTCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TAT

Last Updated on 06.12.2018 16:11