Sequences from confirmation amplicon


Confirmation sequences for line 167D04 and hit At3g04050 are shown.
Line ID 167D04
Locus At3g04050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1049827
Primers used for PCR MS18 - 8474, show primer details
   
Sequence Name 48-K016393-022-167-D04C-8409
GenBank Accession LN506358 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K016393-022-167-D04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAAAAAACAAACAATGATCAGCAATTGT
ATAACTTTGGATCCCTCAAACAAAGACGAAGATCGTCTGTACTCTCGGACCGGCTTCGAG
ATCGGTTGAGATGGTTGAGAAGCTTCTCAAAGCCGGTATGAACGTAGCCCGGTTCAACTT
CTCCCATGGTTCTCACTCATACCATCAAGAAACTCTTGACAATCTCAGAACCGCCATGGA
GAACACTTGTATTCCCTGTGCCGTCATGCTCGACACCAAGGTAACCTAATTTTGAACCGT
CTTTTCAACTTCTCCTTATGTTGTTTTAAGATTCTATTAGGGTCTAGGGACATAATCTAA
CTCGGTTTAATCTTTTCTGGTTTATAGGGTCCTGAGATCCGAACCGGGTTTCTCAAAGAA
GGCAAACCGGTTGAGCTAATTCAAGGTCAAGAGATTACAATCTCAACTGATTACACCATG
GAAGGAGATTC
   
Sequence Name 49-K016393-022-167-D04C-MS18
GenBank Accession LN506663 [GenBank]
Sequencing primer gene specific primer, MS18: 5'-GATTGTGTTTGAATCTCCTTCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K016393-022-167-D04C-ms18
CAGTTGAGATTGTAATCTCTTGACCTTGAATTAGCTCAACCGGTTTGCCTTCTTTGAGAA
ACCCGGTTCGGATCTCAGGACCCTATAAACCAGAAAAGATTAAACCGAGTTAGATTATGT
CCCTAGACCCTAATAGAATCTTAAAACAACATAAGGAGAAGTTGAAAAGACGGTTCAAAA
TTAGGTTACCTTGGTGTCGAGCATGACGGCACAGGGAATACAAGTGTTCTCCATGGCGGT
TCTGAGATTGTCAAGAGTTTCTTGATGGTATGAGTGAGAACCATGGGAGAAGTTGAACCG
GGCTACGTTCATACCGGCTTTGAGAAGCTTCTCAACCATCTCAACCGATCTCGAAGCCGG
TCCGAGAGTACAGACGATCTTCGTCTTTGTTTGAGGGATCCAAAGTTATACAATTGCTGA
TCATTGTTTGTTTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACACTTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGA

Last Updated on 06.12.2018 16:11