Sequences from confirmation amplicon


Confirmation sequences for line 170G03 and hit At4g21120 are shown.
Line ID 170G03
Locus At4g21120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11270907
Primers used for PCR RK12 - 8474, show primer details
   
Sequence Name 43-K015846-022-170-G03C-8409
GenBank Accession LN504658 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K015846-022-170-G03C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTAAGATGCGTCTTTA
GTTCAAAGATGTAAAAATGAAATTTTAAACTTGTAAATTTTAATATTTTTCAACTTTTTC
AAATTAGAAATTCTCTAACAAGAAAATATAAATATAACATACCAAAAAAAAAACGAAAAT
ATAAATATAAAGGAGAAACAGAGGAAATGATAATTTTGTTACCTGCGACGGGAATCTCAA
CGGCGAACTCGGGGTAACAAAAGACGGAGAGCATGGCGGAGACACCAGAGACAACGTAAG
ACAACACGACGGCTGGACCGGAATGATTACGTGCTTCGAGTCCAGGGAGAACGAATATCC
CAGAGCCGATGACTGCACCGATACCAAACCACATGAGATCCCACCACGTCAACGTCTTCT
TCATCTCGGGACCACTACGAGCCTTCATCTCGTTAATCTCGTCGGAGTCTAGGGACCGAG
TCATAATACGATCC
   
Sequence Name 44-K015846-022-170-G03C-RK12
GenBank Accession LN505018 [GenBank]
Sequencing primer gene specific primer, RK12: 5'-GAGTCGGTTCATGGATCGTATTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K015846-022-170-G03C-rk12
GACTCGGTCACTAGACTCCGACGAGATTAACGAGATGAAGGCTCGTAGTGGTCACGAGAT
GAAGAAGACGTTGACGTGGTGGGATCTCATGTGGTTTGGTATCGGTGCAGTCATCGGCTC
TGGGATATTCGTTCTCACTGGACTCGAAGCACGTAATCATTCCGGTCCAGCCGTCGTGTT
GTCTTACGTTGTCTCTGGTGTCTCCGCCATGCTCTCCGTCTTTTGTTACACCGAGTTCGC
CGTTGAGATTCCCGTCGCAGGTAACAAAATTATCATTTCCTCTGTTTCTCATTTATATTT
ATATTTTCGTTTTTTTTTTGGGATGTTATATTTATATTTTCTTGTTAGAGAATTTCTAAT
TTGAAAAAGTTGAAAAATATTAAAATTTACAAGTTTAAAATTTCATTTTTACATCTTTGA
ACTAAAGACGCATCTTAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTATTATAAAATGAAAGTACTTTT
TGATAAA

Last Updated on 06.12.2018 16:11