Sequences from confirmation amplicon


Confirmation sequences for line 171F07 and hit At3g30300 are shown.
Line ID 171F07
Locus At3g30300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11923673
Primers used for PCR NR38 - 8474, show primer details
   
Sequence Name 06-K104193-0022-171-F07C-NR38
GenBank Accession LN488790 [GenBank]
Sequencing primer gene specific primer, NR38: 5'-CACAGATGACTCAATCTCCGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K104193-0022-171-F07C-NR38
TATCTTCGACAATCCCAGATTTGCCCGTCATGTAAAAATCTCACCTTTCTCTTTTGTTTA
GTGGATCTTGAAATTTTATTTTATTTCATCAAGTGGGCCAATATAGAATCGTTTCTTCTT
TCCTTAAACAAAAAGTTTTACTTATGATGACTCTAAAAAGTTTATCAATTGGTTTATGTT
ACAGACACCATTGTATAGAAGACTTTGGGGACCAACAAGACATGTAGAGACATTACTTCC
TGATGCTAATCCTAGAGGTTTTCATTCAGGTTAGGACAGTTCATGTTTTTTTTCCTACAT
TGTCGGATCTGTTACTGAATCTTTTAGATTGTATTGTCTTAAGTTGAATTTAGATTGATG
TTCTGTTTTTGAATTGTGTAACAGATCCTCCTGCAAGGACAAATGGTTTTGTTTTTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 22-K104194-0022-171-F07C-8409
GenBank Accession LN496090 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K104194-0022-171-F07C-8409
TCGGACTGAGCCATTTACAAAACAAAACCATTTGTCCTTGCAGGAGGATCTGTTACACAA
TTCAAAAACAGAACATCAATCTAAATTCAACTTAAGACAATACAATCTAAAAGATTCAGT
AACAGATCCGACAATGTAGGAAAAAAAACATGAACTGTCCTAACCTGAATGAAAACCTCT
AGGATTAGCATCAGGAAGTAATGTCTCTACATGTCTTGTTGGTCCCCAAAGTCTTCTATA
CAATGGTGTCTGTAACATAAACCAATTGATAAACTTTTTAGAGTCATCATAAGTAAAACT
TTTTGTTTAAGGAAAGAAGAAACGATTCTATATTGGCCCACTTGATGAAATAAAATAAAA
TTTCAAGATCCACTAAACAAAAGAGAAAGGTGAGATTTTTACATGACGGGCAAATCTGGG
ATTGTCGAAGATAGGTCTCCAAGAGAAGATAGTGACAGGGATAGAGTAATCGGAGATTGA
GTCATCTGTG

Last Updated on 06.12.2018 16:11