Sequences from confirmation amplicon


Confirmation sequences for line 173H06 and hit At2g31960 are shown.
Line ID 173H06
Locus At2g31960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13590054
Primers used for PCR VL56 - 8474, show primer details
   
Sequence Name 19-K024207-022-173-H06C-VL56
GenBank Accession LN494592 [GenBank]
Sequencing primer gene specific primer, VL56: 5'-AATCTACCAGCAAGAAAAAGGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K024207-022-173-H06C-VL56
CCAGTGAGTCAATAAAGAATATCAAAATTTTTTATTAACAGCTTTCATACTACAAATAAT
ATCAATAAAGAATATCAAAATTTCCACGAAAGCATGGGATAAGATCAGAAGTAAGAGACT
TAAATACTACAAAATATAATCTAGAATCAGGTCACCTCATCAGCAACTTCCACATCTTCT
GTCTGATTAACAGCTTTCAAGACTTCAAAGAGAACAGCAGCAGTTTGATATGCCTTTGTA
AGTTGAGCACTACAAGTAAAACAAATTTGTATTATGAATGAGAATCACAGAAAGATAGAT
TGCAATAGAGATAAGACAGGGATGTCAACAAACCGGTCAGCTTTGTCAGCGGCGTTTTGC
AAAGCTTGGATGTATTTCTTGTAGTAGTGTTGATAGAAACTTTGCATTTCGCGTGCATCA
CTCTTCTGCACGCATGTCGACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATANACAAATTATTC
   
Sequence Name 20-K024207-022-173-H06C-8409
GenBank Accession LN495046 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K024207-022-173-H06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGTCGACATGCGTGCAGAAGAGTGATGCA
CGCGAAATGCAAAGTTTCTATCAACACTACTACAAGAAATACATCCAAGCTTTGCAAAAC
GCCGCTGACAAAGCTGACCGGTTTGTTGACATCCCTGTCTTATCTCTATTGCAATCTATC
TTTCTGTGATTCTCATTCATAATACAAATTTGTTTTACTTGTAGTGCTCAACTTACAAAG
GCATATCAAACTGCTGCTGTTCTCTTTGAAGTCTTGAAAGCTGTTAATCAGACAGAAGAT
GTGGAAGTTGCTGATGAGGTGACCTGATTCTAGATTATATTTTGTAGTATTTAAGTCTCT
TACTTCTGATCTTATCCCATGCTTTCGTGGAAATTTTGATATTCTTTATTGATATTATTT
GTAGTATGAAAGCTGTTAATAAAAAATTTTGATATTCTTTATTGACTCACTGGGTTTTGT
TCATCCTTTTTCTTG

Last Updated on 06.12.2018 16:11