Sequences from confirmation amplicon


Confirmation sequences for line 175G01 and hit At1g53420 are shown.
Line ID 175G01
Locus At1g53420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19927163
Primers used for PCR NH57 - 8474, show primer details
   
Sequence Name 19-K102590-0022-175-G01C-8409
GenBank Accession LN494711 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K102590-0022-175-G01C-8409
TGAAGCATTGGATATGGCCTCTCATATTTTAGACTCCGGAGCCATGTCACCACTGCATAA
ATGTTGCAAAAAGATGATGTGCTATAACTTATAAGCAAATCTCACATGACATATAAACTT
TTATCATTGAATATTGATTTTGTGACTTACAATGTTCCAGCGATTCGAGTGCTAATGTGA
GTGCTGTCCTCTTCATCAAGCTTGGCAAGACCAAAGTCTGATATTTTCGGATTCAGTTGT
TTGTCTAGCAACACATTAGTGGCTTTGATGTCTCTGTGTACAATCTTTAGCCTCGATTCT
TCGTGTAGATATGCTAGCCCTCTCGCCACTCCGATACAGATCTTCCGTCTTGTAGGCCAG
TCCAATCTCAACTGAGTTTCTTGAGGACCTTTATCAAGAAAGGGAAAACGAATCATAACT
AAATTCAGTTTTGACGGTTTATTTAAGGAGCTAGAGTGTTGCTAACCAAACAATGCTCGA
GCTAGACTGTTGTTTTCTACAAACTCATAGAGG
   
Sequence Name 19-K102591-0022-175-G01C-NH57
GenBank Accession LN494712 [GenBank]
Sequencing primer gene specific primer, NH57: 5'-TTATTTGATGGAACAATAATCGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K102591-0022-175-G01C-NH57
AGGACAAACAGGGACCGCGAGTTCTTGAACGAGATTGGCATGATTTCAGCTCTGCACCAC
CCTAATCTGGTTAAACTATATGGATGTTGCGTTGAAGGAGGCCAACTTTTATTAGTCTAT
GAGTTTGTAGAAAACAACAGTCTCGCTCGAGCATTGTTTGGTTAGCAACACTCTAGCTCC
TTAAATAAACCGTCAAAACTGAATTTAGTTATGATTTGTTTTCCCTTTCTTGATAAAGGT
CCTCAAGAAACTCAGTTGAGATTGGACTGGCCTACAAGACGGAAGATCTGTATCGGAGTG
GCGAGAGGGCTAGCATATCTACACGAAGAATCGAGGCTAAAGATTGTACACAGAGACATC
AAAGCCACTAATGTGTTGCTAGACAAACAACTGAATCCGAAAATATCAGACTTTGGTCTT
GCCAAGCTTGATGAAGAGGACAGCACTCACATTAGCACTCGAATCGCTGGAACATTGTAA
GTCACAAAATCAATATTCAATGATAAAAGTTTATATGTCATGTGAGATTTGCTTATAAGT
TATAGCACATCATCTTTTTGCAACATTTATGCAGTGGTTACATGGCTCCGGAGTCTAAAA
TATGAGAGGCCATATCCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGGAATCTTTATT
TCGACGTGTCTACA
Primers used for PCR MN53 - 8474, show primer details
   
Sequence Name 37-K118534-0022-175-G01C-MN53
GenBank Accession LN502789 [GenBank]
Sequencing primer gene specific primer, MN53: 5'-TCTCTTGCCCTATCATTTCGTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118534-0022-175-G01C-MN53
TTCAGTCTCTTTGTTTCTCTATGGACTGAAGCTTCTTCAAGTTTCTCCACCTCCACCATC
TTCTTACCTTCTAACATCTTCACCACTTCTGACATTGACGGTCTCTCACAAGGTTCTGAG
CTTGTACACATTATCGCAATTTGGATCATGGTCATTGCTTCTTCTCTGTTATATTCTGAT
CCTAGTCTTGGATCCACCAGTTCCAACAGATTATTCTTCTCTCTCAAAACCTCCACCTAG
GGAAACAAGAACAAAATATAGTTGAGATCTTTTGTTCAATAGTTCTGTGAAATGTAATCA
ATCAAAAAGATCATTACCCAGTCAATAAGATAGAAGGTATTGTTTTTGGATCGTTCTATT
TTATTGCTTCTTCCATGAACAATCTCTAGAGCTACTATACCAAAGCTGTAAACGTCAGCT
TTATCTGTCAAAATTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 16-K118533-0022-175-G01C-8409
GenBank Accession LN493634 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118533-0022-175-G01C-8409
ATTTACATTGAATATATCCAATTTTGACAGATAAAGCTGACGTTTACAGCATTTGGTATA
GTAGCTCTAGAGATTGTTCATGGAAGAAGCAATAAAATAGAACGATCCAAAAACAATACC
TTCTATCTTATTGACTGGGTAATGATCTTTTTGATTGATTACATTTCACAGAACTATTGA
ACAAAAGATCTCAACTATATCTTGTTCTTGTTTCCCTAGGTGGAGGTTTTGAGAGAGAAG
AATAATCTGTTGGAACTGGTGGATCCAAGACTAGGATCAGAATATAACAGAGAAGAAGCA
ATGACCATGATCCAAATTGCGATAATGTGGACTAGCTCATAACCTTGTGAGAGACCGTCA
ATGTCAGAAGTGGTGAAGATGTTACAAGGTAACAAGATGGTGGAGGTGGAGAAACTTGAA
GAAGCTTCGGTCCATAGAGAAACAAAGAGACTTGAAAACATGAACACTATGAATAAATAC
TACAAGATGATAGGGCTAGATAAGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCACAATT

Last Updated on 06.12.2018 16:11