Sequences from confirmation amplicon


Confirmation sequences for line 176D07 and hit At1g71680 are shown.
Line ID 176D07
Locus At1g71680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26944926
Primers used for PCR RK26 - 8474, show primer details
   
Sequence Name 29-K015895-022-176-D07C-8409
GenBank Accession LN499070 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K015895-022-176-D07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCNCTGGAAAAATAACAATAA
CAATCACCGTCAAATGGATTACAACGATTGGTTACCAGTCACAGCGTCTAGAGAAGCCAA
ATGGTATTACTCCGCCTTCCACAATGTCACGGCCATGGTTGGCGCCGGAGTTCTTGGTCT
CCCTTTCGCCATGTCACAACTTGGCTGGTAATTTTTTAAGAAATGTTTAAATTTGTCTGA
TCTTAACTTCACAAAATAAAGTCGTAATTTAACGATAAATTTGTTGCTAACAGGGGACCT
GGACTTGTTGCTATAATAATGTCATGGGCTATAACGTTCTACTCGTTATGGCAAATGGTA
CAGTTGCACGAAGCCGTTCCAGGGAAACGGTTGGACCGTTACCCCGAGCTAGGGCAAGAA
GCTTTTGGACCAAAACTAGGTTACTGGATCGTCATGCCGCAACAGCTTCTTGTCCAAATC
GCTTCCGACATAGTCTACAACGTGACAGGAGGAAAATCTCTCAAGAAATTTGTCGAGCTT
CTATTTCCGAATCTCGAACATATCCGTCAGACTTATTACATCCTTGGATTCGCCGCGCTT
CA
   
Sequence Name 30-K015895-022-176-D07C-RK26
GenBank Accession LN499506 [GenBank]
Sequencing primer gene specific primer, RK26: 5'-AGATTGAGAGAGGACAAGCTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K015895-022-176-D07C-rk26
GCGGCGAATCCAAGGATGTAATAAGTCTGACGGATATGTTCGAGATTCGGAAATAGAAGC
TCGACAAATTTCTTGAGAGATTTTCCTCCTGTCACGTTGTAGACTATGTCGGAAGCGATT
TGGACAAGAAGCTGTTGCGGCATGACGATCCAGTAACCTAGTTTTGGTCCAAAAGCTTCT
TGCCCTAGCTCGGGGTAACGGTCCAACCGTTTCCCTGGAACGGCTTCGTGCAACTGTACC
ATTTGCCATAACGAGTAGAACGTTATAGCCCATGACATTATTATAGCAACAAGTCCAGGT
CCCCTGTTAGCAACAAATTTATCGTTAAATTACGACTTTATTTTGTGAAGTTAAGATCAG
ACAAATTTAAACATTTCTTAAAAAATTACCAGCCAAGTTGTGACATGGCGAAAGGGAGAC
CAAGAACTCCGGCGCCAACCATGGCCGTGACATTGTGGAAGGCGGAGTAATACCATTTGG
CTTCTCTAGACGCTGTGACTGGTAACCAATCGTTGTAATCCATTTGACGGTGATTGTTAT
TGTTATTTTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATT

Last Updated on 06.12.2018 16:11