Sequences from confirmation amplicon


Confirmation sequences for line 181G11 and hit At1g01070 are shown.
Line ID 181G11
Locus At1g01070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:40397
Primers used for PCR BC33 - 8474, show primer details
   
Sequence Name 15-K103979-0022-181-G11C-BC33
GenBank Accession LN492945 [GenBank]
Sequencing primer gene specific primer, BC33: 5'-CTTTCCTGGCGTTACAAAATAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103979-0022-181-G11C-BC33
TTTCGCATCTTCCTCAAGTTTAAAAAATGCGACGTATAGCTAAGAAAAGACCTTTTTTCA
GGAAAAGCTTTAGAAAGAGGGCTCAGTGGATGCAGTTACGTATACTTAAGGTAATTTGAT
GGAAAAGAAAACCAATCAGATTGCATTACAATTAATAAGAAAATATAATGTTCTATATTA
TGTTGTCAATACTTTCAGTAGTGCTTTTTAAAAATCTAGATTAATATATATTATCAATTG
ATGATAAAGAGGTTAGATGGTTCCATCGTTACCTGAAAATAAGGGCCAAAGCGAAGGTGA
TTGCAGGCAACATGCTTACCAAAGCACACGAAACAGTTGCTGACGTGTACGACAGACCAA
GCAAAAAGAAAAACTGCATCAAACTCGCCCTGTCAATTTAAACCCATAAATTAATTAAAA
GATCTTTAATAATTTAGCTTATGATTAGTACAAACTAAAATGCAATTATAAATAATATAA
ATTGACCATTGATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCG
   
Sequence Name 31-K103980-0022-181-G11C-8409
GenBank Accession LN500135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K103980-0022-181-G11C-8409
GAGCCATTTATCAATGGTCAATTTATATTATTTATAATTGCATTTTAGTTTGTACTAATC
ATAAGCTAAATTATTAAAGATCTTTTAATTAATTTATGGGTTTAAATTGACAGGGCGAGT
TTGATGCAGTTTTTCTTTTTGCTTGGTCTGTCGTACACGTCAGCAACTGTTTCGTGTGCT
TTGGTAAGCATGTTGCCTGCAATCACCTTCGCTTTGGCCCTTATTTTCAGGTAACGATGG
AACCATCTAACCTCTTTATCATCAATTGATAATATATATTAATCTAGATTTTTAAAAAGC
ACTACTGAAAGTATTGACAACATAATATAGAACATTATATTTTCTTATTAATTGTAATGC
AATCTGATTGGTTTTCTTTTCCATCAAATTACCTTAAGTATACGTAACTGCATCCACTGA
GCCCTCTTTCTAAAGCTTTTCCTGAAAAAATGTCTTTTCTTAGCTATACGTCGCATTTTT
ATAAACTTTGATGGAATGATGCGGAAAATTATTAAAAAGCGACTCTATTTTGTA

Last Updated on 06.12.2018 16:11