Sequences from confirmation amplicon


Confirmation sequences for line 182H05 and hit At3g47110 are shown.
Line ID 182H05
Locus At3g47110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17348162
Primers used for PCR NH56 - 8474, show primer details
   
Sequence Name 23-K102590-0022-182-H05C-8409
GenBank Accession LN496506 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K102590-0022-182-H05C-8409
AGCTCATCATAACTTATCTTTTCATAAAACGATTTCACTGGGGAGAAAGACCGATCATTC
TCATTATTATTAGCTCTGACACTCTTCACTCTCAGTTTGTACCAGCATAGATAAACCACA
CACAGACATAGAAGCAAAAGTGCTGCCATAACTGCACTGACACAAATGGTGATTATCTTT
CTGACTGATGAATGCCTTCTTGGTAATTCAACAGAGCATGGCTGAAGCTGTAATGACGGG
ATGCCTCCACAGAGGTTTATGTTACCAAAGACCGACATCGCACTGGTATTACGAAATACC
CCTTCCGTTGGCACAGCTCCGTCGAAGTTGTTCAAAGAGAGATTCAGGTTCTGCAGTTTG
GAAAAGTTCGCCATATATTCAGGTATTGTACCAGAGAGATTATTCTTCGACAGATCTAGG
AATCTGAGGCCAGTCAACCCTCTTAACNTGGGGAATGG
   
Sequence Name 23-K102591-0022-182-H05C-NH56
GenBank Accession LN496507 [GenBank]
Sequencing primer gene specific primer, NH56: 5'-CCATTCCAGATATAAGAGGGTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K102591-0022-182-H05C-NH56
TCTCTCTGGTACAATACCTGAATATATGGCGAACTTTTCCAACTGCAGAACCTGAATCTC
TCTTTGAACAACTTCGACGGAGCTGTGCCAACGGAAGGGGTATTTCGTAATACCAGTGCG
ATGTCGGTCTTTGGTAACATAAACCTCTGTGGAGGCATCCCGTCATTACAGCTTCAGCCA
TGCTCTGTTGAATTACCAAGAAGGCATTCATCAGTCAGAAAGATAATCACCATTTGTGTC
AGTGCAGTTATGGCAGCACTTTTGCTTCTATGTCTGTGTGTGGTTTATCTATGCTGGTAC
AAACTGAGAGTGAAGAGTGTCAGAGCTAATAATAATGAGAATGATCGGTCTTTCTCCCCA
GTGAAATCGTTTTATGAAAAGATAAGTTATGATGAGCTCTACAAGACAACCGGTGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAA
Primers used for PCR MN52 - 3144, show primer details
   
Sequence Name 06-K102864-0022-182-H05C-3144
GenBank Accession LN488762 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K102864-0022-182-H05C-3144
TCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTTGGA
CCCAAGAAATCCTTTAAACACAGCCCCAAAGTTACCCGACCCAATCAAATTGCTGGAAGT
TTTGAATCTCTGCAAACGTGGTGCTGCAAAGAGTTTCATAGCGGAATGTGAAGCATTAGG
AGGTATAAGGCATCGCAACCTTGTGAAGTTGGTTACTATCTGTTCAAGTTCTGATTTTGA
AGGAAATGATTTTAGAGCTCTAGTGTATGAGTTCATGCCTAATGGAAACTTGGACATGTG
GCTGCACCCGGATGAAATAGAAGAAACTGGCAATCCCTCGAGAACCCTGGGCCTTTTTGC
GAGACTCAACATTGCCATAGATGTTGCTTCGGCTCTGGTTTACNTTCATAC
   
Sequence Name 06-K102869-0022-182-H05C-MN52
GenBank Accession LN488763 [GenBank]
Sequencing primer gene specific primer, MN52: 5'-AGTATGAAGATAAACCAGAGCCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K102869-0022-182-H05C-MN52
CTTCTATTTCATCCGGGTGCAGCCACATGTCCAAGTTTCCATTAGGCATGAACTCATACA
CTAGAGCTCTAAAATCATTTCCTTCAAAATCAGAACTTGAACAGATAGTAACCAACTTCA
CAAGGTTGCGATGCCTTATACCTCCTAATGCTTCACATTCCGCTATGAAACTCTTTGCAG
CACCACGTTTGCAGAGATTCAAAACTTCCAGCAATTTGATTGGGTCGGGTAACTTTGGGG
CTGTGTTTAAAGGATTTCTTGGGTCCAAAACACTGATAGTTTAAACCGAAGGCGGGAAAC
GACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAG
GAAGGGTCTTGCGAACGATAGTGGGATTGTGCGTCATCCCCTACGTCATTGGAGATATCA
CATCATTCTAATTGCTTTGA

Last Updated on 06.12.2018 16:11