Sequences from confirmation amplicon


Confirmation sequences for line 187A04 and hit At5g63680 are shown.
Line ID 187A04
Locus At5g63680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25491749
Primers used for PCR KT24 - 8474, show primer details
   
Sequence Name 48-K100814-0022-187-A04C-8409
GenBank Accession LN506440 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K100814-0022-187-A04C-8409
GCCATTTCCCATCCCAAGATTGTCGAAATTCAGGACTCCTTCTTGGTTCTCAACCTTAAT
AAGATAAAAATTCTAATCACAATCCTACCTTTAAAACCAACTTTAGCCACAAGTGAAATA
AGGTGTAACTTTGTACCTTCGACATCAACATTATACTCTTGGAATGTGATCCAAGGACTT
TCCGGACATTAACAAGATCTGAACCTTTGCGAACAAATGAAAGAGCAATCATATCGATGT
TGTTTGGAACACCCCATTTAAGAATATCTTCTACATCCTTGTCAGTCAATGTGGGGAGAT
CAACAACAACACCTGGGAGATTCACATTTTTTCTTTCACCTAGCATGGCTGTATTTTCAC
ATCGACATATAACAGTCCCAGCGTTTGGATCACATGACACGACAGCAAGACTTATGCTTC
CTNTTGCACAT
   
Sequence Name 48-K100826-0022-187-A04C-KT24
GenBank Accession LN506442 [GenBank]
Sequencing primer gene specific primer, KT24: 5'-TATGTGCAGATGGAAGCATAAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K100826-0022-187-A04C-KT24
GTCGATGTGAAAATACGCCATGCTAGGTGAAAGAAAAAATGTGAATCTCCCAGGTGTTGT
TGTTGATCTCCCCACATTGACTGACAAGGATGTAGAAGATATTCTTAAATGGGGTGTTCC
AAACAACATCGATATGATTGCTCTTTCATTTGTTCGCAAAGGTTCAGATCTTGTTAATGT
CCGGAAAGTCCTTGGATCACATTCCAAGAGTATAATGTTGATGTCGAAGGTACAAAGTTA
CACCTTATTTCACTTGTGGCTAAAGTTGGTTTTAAAGGTAGGATTGTGATTAGAATTTTT
ATCTTATTAAGGTTGAGAACCAAGAAGGAGTCCTGAATTTCGACAATCTTGGGATGGGAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11