Sequences from confirmation amplicon


Confirmation sequences for line 210C09 and hit At3g48520 are shown.
Line ID 210C09
Locus At3g48520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17975432
Primers used for PCR MS54 - 8474, show primer details
   
Sequence Name 25-K017179-022-210-C09C-MS54
GenBank Accession LN497224 [GenBank]
Sequencing primer gene specific primer, MS54: 5'-TATTAGTTTTATCATGGCGGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K017179-022-210-C09C-MS54
AACGTCAGCAGCGATGACGTGGCTGTTTTGGTTGTTGACTGAGAACGATGACGTGGAGCG
GAAGATTTTAGAAGAAGTGGACCCGTTGGTGAGTTTAGGGTTAGGGTTTGAGGATTTGAA
AGAGATGGCTTACACGAAGGCTTGTCTATGTGAAGCCATGAGGCTATATCCTCCAGTTTC
GTGGGACTCTAAACATGCTGCAAACGACGACGTTTTGCCTGATGGGACACGTGTCAAGAG
AGGAGACAAAGTGACCTATTTCCCATACGGTATACGAATTAGAATCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACG
   
Sequence Name 29-K017179-022-210-C09C-8409
GenBank Accession LN499076 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K017179-022-210-C09C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGATTCTAATTCGTATACCGTATGGGAA
ATAGGTCACTTTGTCTCCTCTCTTGACACGTGTCCCATCAGGCAAAACGTCGTCGTTTGC
AGCATGTTTAGAGTCCCACGAAACTGGAGGATATAGCCTCATGGCTTCACATAGACAAGC
CTTCGTGTAAGCCATCTCTTTCAAATCCTCAAACCCTAACCCTAAACTCACCAACGGGTC
CACTTCTTCTAAAATCTTCCGCTCCACGTCATCGTTCTCAGTCAACAACCAAAACAGCCA
CGTCATCGCTGCTGACGTTGTATCTCTTCCCGCCATGATAAAACTAAT

Last Updated on 06.12.2018 16:11