Sequences from confirmation amplicon


Confirmation sequences for line 216E07 and hit At2g02560 are shown.
Line ID 216E07
Locus At2g02560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:689809
Primers used for PCR CK66 - 8474, show primer details
   
Sequence Name 11-K024722-022-216-E07C-8409
GenBank Accession LN490942 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K024722-022-216-E07C-8409
CCGGACATGAAGCCATTGACCATCTCCTCCATCTTTTTTTACTTTCCTCAGAGCTCTCTC
CTCTCCGCAACTTCCACCACCACCAAATCGGAACTCTCGGCGTTCTACTCCGTCTCCGGC
GTTACCTCCGTCGATTTCATCTTCTCAGGTTACTTAGGGTTACCTCTTTTTTGCTTTTTG
ATCGATAAAGTGTGATGATGTCTAGCGTTAACTCTTTTCGTCTTCGTAGTCGTAGTCTTA
GGGTTTTGGTGGCGACGATACTTTGATTTATCAAACCGAACTTCTTCTTTGTGATCTTCG
ATGAATCAAAAGTTTTCTAAGGGATATTAATTAGGTTTTGTTACCGGCGATAATTAATCC
ATCTTCGATGCTTCTTCTTTACACTTCTGACTTTGTTTCTGATGTTTTAACCTCTGTGGA
GAGCTTGATAGAGTG
   
Sequence Name 12-K024722-022-216-E07C-CK66
GenBank Accession LN491401 [GenBank]
Sequencing primer gene specific primer, CK66: 5'-CAGAGCAATGTCACTCTATCAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K024722-022-216-E07C-CK66
CACAGAGGTTAAAACATCAGAAACAAAGTCAGAAGTGTAAAGAAGAAGCATCGAAGATGG
ATTAATTATCGCCGGTAACAAAACCTAATTAATATCCCTTAGAAAACTTTTGATTCATCG
AAGATCACAAAGAAGAAGTTCGGTTTGATAAATCAAAGTATCGTCGCCACCAAAACCCTA
AGACTACGACTACGAAGACGAAAAGAGTTAACGCTAGACATCATCACACTTTATCGATCA
AAAAGCAAAAAAGAGGTAACCCTAAGTAACCTGAGAAGATGAAATCGACGGAGGTAACGC
CGGAGACGGAGTAGAACGCCGAGAGTTCCGATTTGGTGGTGGTGGAAGTTGCGGAGAGGA
GAGAGCTCTGAGGAAAGTAAAAAAAGATGGAGGAGATGGTCAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11