Sequences from confirmation amplicon


Confirmation sequences for line 218D01 and hit At1g68400 are shown.
Line ID 218D01
Locus At1g68400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25648204
Primers used for PCR PP19 - 8474, show primer details
   
Sequence Name 12-K102660-0022-218-D01C-8409
GenBank Accession LN491526 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K102660-0022-218-D01C-8409
GCCATTTACATTGTAAATGGAGAGTTTCGTGCATTTCAGCAATGGAGCTCCACAGAGAGA
TGGGTTTTGTGTAAAGACTGATTCAGGGAATTGAGACAATGAATTTGGTATCTGACCGTT
AAAGTTGTTACCGGAGACGTTGAAGTCTTGTAAATCGGAGAGGTTGATGTTCGGTATTTG
ACCGGAGAACCGGTTTGATTCTAACCGGAGAGTGAGGAGATGGGTTAAATCGGTTAAATC
CGGTGGAATCTGGCCGGAGAAGTTGTTGAAAGAGAGATCAAGACGGTAGAGACGAGTGAG
TGAAGTGATTGAAGTGGGGAAGTTACCGGAGAATTGGTTGTTTGATAAAAAGAGAAGCTT
TAAAGCTGTGAGGTTCGAGAGGTTTGGGATTGGACCAGAGAGATTGTTATGTTTCAGACT
GAGAACTCTGAGTGAGTGGAGTGAGG
   
Sequence Name 31-K102661-0022-218-D01C-PP19
GenBank Accession LN500113 [GenBank]
Sequencing primer gene specific primer, PP19: 5'-CCTCACTCACATCACTCAGAGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K102661-0022-218-D01C-PP19
ATCTCTCGGTCCATCCCAACCTCTCGAACTCAAGCTTTAAGCTTCTCTTTTTATCAAACA
ACCAATTCTCCGGTAACTTCCCCACTTCAATCACTTCACTCACTCGTCTCTACCGTCTTG
ATCTCTCTTTCAACAACTTCTCCGGCCAGATTCCACCGGATTTAACCGATTTAACCCATC
TCCTCACTCTCCGGTTAGAATCAAACCGGTTCTCCGGTCAAATACCGAACATCAACCTCT
CCGATTTACAAGACTTCAACGTCTCCGGTAACAACTTTAACGGTCAGATACCAAATTCAT
TGTCTCAATTCCCTGAATCAGTCTTTACACAAAACCCATCTCTCTGTGGAGCTCCATTGC
TGAAATGCACGAAACTCTCCATTTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACAT
Primers used for PCR MN56 - 3144, show primer details
   
Sequence Name 07-K102864-0022-218-D01C-3144
GenBank Accession LN489224 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K102864-0022-218-D01C-3144
TTCGCAGACCCGATGAAGCAAGGCTTCTCCTTTAAACAAGCCGGAAACTGTACCTTCATC
TCCAACTTCAATTCACGGCGGCGACAAAAGCAACAACACATCAAGAATCAGCACCATCTC
ACTTATAGCTATCATCCTCGGAGACTTTATTATCTTAAGCTTTGTCTCTCTTCTTCTCTA
CTACTGTTTCTGGAGACAATACGCAGTGAACAAGAAGAAACACTCGAAGATTCTTGAAGG
AGAAAAGATTGTCTACTCATCAAATCCTTACCCAACATCAACGCANAACAACAACAATCA
GAATCAACAAGTTGGTGATAAGGGTAAAATGGTCTTTTTCGAAGGAACTAGACGTTTTGA
GTTAGAAGATCTTCTCCGAGCATCGGCGGAGATGTTAGGGAAGGGAGGATT
   
Sequence Name 07-K102869-0022-218-D01C-MN56
GenBank Accession LN489225 [GenBank]
Sequencing primer gene specific primer, MN56: 5'-GAATCCTCCTTTCCCTAACATCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K102869-0022-218-D01C-MN56
TACTCAAAGTCTAGTTCCTTCGAAAAGACCATTTTACCCTTATCACCCATTTGTTGATTC
TGATTGTTGTTGTTTTGCGTTGATGTTGTGGTAAGGATTTGATGAGTAGACAATCTTTTC
TCCTTCAAGAATCTTCGAGTGTTTCTTCTTGTTCACTGCGTATTGTCTCCAGAAACAGTA
GTAGAGAAGAAGAGAGACAAAGCTTAAGATAATAAAGTCTCCGAGGATGATAGCTATAAG
TGAGATGGTGCTGATTCTTGATGTGTTGTTGCTTTTGTCGCCGCCGTGAATTGAAGTTGG
AGATGAAGGTACAGTTTCCGGCTTGTTTAAAGGAGAAGCCTTTGCTTCATCGGGTCTTGC
GAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACT
TGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGAGGGGGT
CCATCTTTGGGAAAA

Last Updated on 06.12.2018 16:11