Sequences from confirmation amplicon


Confirmation sequences for line 219E09 and hit At1g08100 are shown.
Line ID 219E09
Locus At1g08100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2528175
Primers used for PCR GL01 - 8474, show primer details
   
Sequence Name 38-K016601-022-219-E09C-8409
GenBank Accession LN502806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K016601-022-219-E09C-8409
TAGATTTCCCGGATATGTAGATTCTATGGTACGCCGTTACAAATTACAGGACTTGGATTT
TCGTTCTTCTGTATGGATATTCCATGGGAGTTGAGTTAAGCACAGACAATGTTATCGCCG
AGTACTTCTTTGATAGGTTTGTTTTCTGTCTCGATTGGAAATTGACAACTTCACATATAT
TCAGGAATAACATAAGAGTAAAATTTATATTCCCTTTTATTTTTATTTTGTCAATCGTTC
CCTTTTATTTAAAATAAAAAAATGGTATGTTCAGGTTTCACTTGAAGCTTCACACAGCGG
GGATTATAGCAGCATGTTTCGGAATGGCCAATTTCTTTGCTCGTCCAGCAGGAGGCTGGG
CATCTGACATTGCAGCCAAGCGCTTCGGAATGCGAGGGAGGTTGTGGACTTTGTGGATCA
TTCAGACGTCCGGTGGTCTCTTTTGTGTGTGGCTCGGACGTGCCAACACCCTCGTCACTG
CCGTTGTATCTATGGTCCTCTTCTCTTT
   
Sequence Name 42-K016601-022-219-E09C-GL01
GenBank Accession LN504281 [GenBank]
Sequencing primer gene specific primer, GL01: 5'-CTTGTGCTCCTAAAGAGAAGAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K016601-022-219-E09C-gl01
CATACATACAACGGCAGTGACGAGGGTGTTGGCACGTCCGAGCCACACACAAAAGAGACC
ACCGGACGTCTGAATGATCCACAAAGTCCACAACCTCCCTCGCATTCCGAAGCGCTTGGC
TGCAATGTCAGATGCCCAGCCTCCTGCTGGACGAGCAAAGAAATTGGCCATTCCGAAACA
TGCTGCTATAATCCCCGCTGTGTGAAGCTTCAAGTGAAACCTGAACATACCATTTTTTTA
TTTTAAATAAAAGGGAACGATTGACAAAATAAAAATAAAAGGGAATATAAATTTTACTCT
TATGTTATTCCTGAATATATGTGAAGTTGTCAATTTCCAATCGAGACAGAAAACAAACCT
ATCAAAGAAGTACTCGGCGATAACATTGTCTGTGCTTAACTCAACTCCCATGGAATATCC
ATACAGAAGAACGAAAATCCAAGTCCTGTAATTTGTAACGGCGTACCATAGAATCTACAT
ATCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11