Sequences from confirmation amplicon


Confirmation sequences for line 219H01 and hit At5g65380 are shown.
Line ID 219H01
Locus At5g65380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26124339
Primers used for PCR 17fs - 8474, show primer details
   
Sequence Name 28-K111325-0022-219-H01C-17fs
GenBank Accession LN498902 [GenBank]
Sequencing primer gene specific primer, 17fs: 5'-ATCTTGCTCCTTTACCATTACCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K111325-0022-219-H01C-17fs
CTACATAGTTCAGTAAATAAGTATCTAGATGTTAATAGTTTTCTTGTATCTAGATGCCCA
CACAAAAATTTAGAATAAAAACAAGTGAACATGAAAAAATTATAAATTAAACAAAACTAT
AATAGCAAGGAGAGTTATCTCTAATTTTCTATTCTCGTATACATCTTAATTCAGACAAGA
CGACAAATATAAAGATAAGAAGAAATCAAGGCAGACACGGAAGAAGATAGCAAAAGCTGT
AAGGATCTAGCCATATATGTGCCAAATGTTAGGTTTCAATTTCCTAAATATTCCCACACA
CAAAATTATTGTTCCTCGAAAATGTAGATGTCCGCAGCGTTATTA

Last Updated on 06.12.2018 16:11