Sequences from confirmation amplicon


Confirmation sequences for line 221F09 and hit At2g47800 are shown.
Line ID 221F09
Locus At2g47800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19579825
Primers used for PCR 14aq - 8474, show primer details
   
Sequence Name 09-K106755-0022-221-F09C-8409
GenBank Accession LN490195 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K106755-0022-221-F09C-8409
TTTACATTGAATATATCCTTGAGTTGACACCGTTCCAGGCTCTGATAAACAGAAAAAACG
AAAAACAGATGTTGTTATATGTAACAAATGGTTTTTAGCTTTAGAAGATCAAGAGGCAAA
ACATGTTTTATCATCACCTTCCAGATTTCTTCGTCAGAGTATTGCTCTGTCGGGTCGATG
TTGCTTCTCACGGTTCCTTCAAAGAGGACAGGTTCTTGCGGAATGATTCCGAATCTTGAC
CTGAGATCATGTAGCCCTAGAGTGCTTATATCAATCCCGTCTATGATTATCTTCCCTCCT
GATGGTTCTACAAGCCTGAACAAGACTTGGATCAATGTCGATTTCCCGCTTCCCGTCCGT
CCAACCACACCAACCTTCTCTCCTCCTTTGATGTCAAGAGTGATCCCCTTGAGCACAAGT
GGAGTGTTCGGTCTGTAGCGCACCTGTTGTTTACAACACACATCATCCTTCACAAGTTTG
AACAGTAAAGGTGAGTTT
   
Sequence Name 25-K106756-0022-221-F09C-14aq
GenBank Accession LN497462 [GenBank]
Sequencing primer gene specific primer, 14aq: 5'-ACAGCCTTGTGTTAAAACTCACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K106756-0022-221-F09C-14aq
GATGATGTGTGTTGTAACAACAGGTGCGCTACAGACCGAACACTCCACTTGTGCTCAAGG
GGATCACTCTTGACATCAAAGGAGGAGAGAAGGTTGGTGTGGTTGGACGGACGGGAAGCG
GGAAATCGACATTGATCCAAGTCTTGTTCAGGCTTGTAGAACCATCAGGAGGGAAGATAA
TCATAGACGGGATTGATATAAGCACTCTAGGGCTACATGATCTCAGGTCAAGATTCGGAA
TCATTCCGCAAGAACCTGTCCTCTTTGAAGGAACCGTGAGAAGCAACATCGACCCGACAG
AGCAATACTCTGACGAAGAAATCTGGAAGGTGATGATAAAACATGTTTTGCCTCTTGATC
TTCTAAAGCTAAAAACCATTTGTTACATATAACAACATCTGTTTTTCGTTTTTTCTGTTT
ATCAGAGCCTGGAACGGTGTCAACTCAAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11