Sequences from confirmation amplicon


Confirmation sequences for line 222B03 and hit At4g01890 are shown.
Line ID 222B03
Locus At4g01890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:817168
Primers used for PCR 10qt - 8474, show primer details
   
Sequence Name 25-K115781-0022-222-B03C-10qt
GenBank Accession LN497597 [GenBank]
Sequencing primer gene specific primer, 10qt: 5'-CTTGTCGCTCTTACCAATACTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115781-0022-222-B03C-10qt
ACTTATTTATTTCTCATGAATATCAAACTTTTGGATAATTGGGTCTAATGGTTTATTTGC
TATTGATTTTTTTTTAAAAACAATAAAGGTTGATGGGACCATCGTGCCACGGGATGGACC
AAAGTCGTGGCCAAGCAGTTTAAACAAAAGACAATGGCTCGCCTTTTATAGAATCAACGG
AATGGCTCTACAAGGCGCCGGAGTTATCGACGGTCGTGGACAAAATTGGTGGGATCTTCC
CTGCAAACCTCATCAGGTAATTAAATACTCACTAGCTAGTACACATTTTCAAATTAATCA
TAATCTAAAAAGTGAAAGATTCGATAATGTAATGTGTGGTTTATATAAGTCATATGACTA
TTTGAAATCAAAATATTTACTATGTAATATTATGATAATATGTTCAAGCAGAATGTCAAC
AAAACTAAACTTGCTGGTCCATGTGAAAGCCCCGCCGTAAGTAAAACCAAACATTTAAAA
GATTATATGTTTCTTACCCTTATTATTCATATTTAATGTTGAATAATGTTCAATGGTTTT
GGTGAGGTTAGGCCTGTCCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 01-K115780-0022-222-B03C-8409
GenBank Accession LN486710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K115780-0022-222-B03C-8409
TCCGGACAGGCCTAACCTCACCAAAACCATTGAACATTATTCAACATTAAATATGAATAA
TAAGGGTAAGAAACATATAATCTTTTAAATGTTTGGTTTTACTTACGGCGGGGCTTTCAC
ATGGACCAGCAAGTTTAGTTTTGTTGACATTCTGCTTGAACATATTATCATAATATTACA
TAGTAAATATTTTGATTTCAAATAGTCATATGACTTATATAAACCACACATTACATTATC
GAATCTTTCACTTTTTAGATTATGATTAATTTGAAAATGTGTACTAGCTAGTGAGTATTT
AATTACCTGATGAGGTTTGCAGGGAAGATCCCACCAATTTTGTCCACGACCGTCGATAAC
TCCGGCGCCTTGTAGAGCCATTCCGTTGATTCTATAAAAGGCGAGCCATTGTCTTTTGTT
TAAACTGCTTGGCCACGACTTTGGTCCATCCCGTGGCACGATGGTCCCATCAACCTTTAT
TGTTTTTAAAAAAAAATCAATAGCAAATAAACCATTAGACCCAATTATCCAAAAGTTTGA
TATTCATGTAGAAATTAAATAAGTTTATTAGTAACTGGTACTTGGAAGTATTG

Last Updated on 06.12.2018 16:11