Sequences from confirmation amplicon


Confirmation sequences for line 224B07 and hit At5g46410 are shown.
Line ID 224B07
Locus At5g46410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18827743
Primers used for PCR CN41 - 8474, show primer details
   
Sequence Name 38-K104161-0022-224-B07C-CN41
GenBank Accession LN502960 [GenBank]
Sequencing primer gene specific primer, CN41: 5'-TATTGTCGGAGTCTGATTTCTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K104161-0022-224-B07C-CN41
GCTCGTTCATTAATCAGTTGTTCCTTAAAATTGATGTTGTATACTGAATCATGTTTAGAA
ACAGAAGGTGAACTGACTTATTTCTTCCTTTTGAGGAAATATGGTTAAGCATTTTTATGT
CTGTGTACTGTTAAATTTTATGTTTCTCTTACCTCTAAGGTAGATGAGCTACTGAGCTTA
CCAAATTCTCTTTTCCTTAAATGCAGAAACTTTGGTCCATTCGACTCTGGAATCGTGTAA
TGTTGCAGATTTTTCCTTCAGAGTCTTTTTCAACATGCAAGAAAACACGGTCTATGTGAG
ACAAAGGCCACACCTTTACAGGTTCTTGGAGAGGGTGGGAGAGTTGTTTCATGTCGTTAT
CTTCACAGCAAGCCACAGCATCTATGCCTCACAACTTCTAGATATACTGGACCCAGATGG
AAAGTTCATATCACAGCGTTTTTATCGCGATTCATGCATTCTTTTGGATGGAATTTACAC
CAAGGATTTAACTGTTCTGGGTCTTGACAACTGTATATAATTGACAATTGTCCGCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCG
   
Sequence Name 38-K104162-0022-224-B07C-8409
GenBank Accession LN502961 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K104162-0022-224-B07C-8409
TTCGGACATGAGCCATTTACAATTGAATATATCCTGCGGACAATTGTCAATTATATACAG
TTGTCAAGACCCAGAACAGTTAAATCCTTGGTGTAAATTCCATCCAAAAGAATGCATGAA
TCGCGATAAAAACGCTGTGATATGAACTTTCCATCTGGGTCCAGTATATCTAGAAGTTGT
GAGGCATAGATGCTGTGGCTTGCTGTGAAGATAACGACATGAAACAACTCTCCCACCCTC
TCCAAGAACCTGTAAAGGTGTGGCCTTTGTCTCACATAGACCGTGTTTTCTTGCATGTTG
AAAAAGACTCTGAAGGAAAAATCTGCAACATTACACGATTCCAGAGTCGAATGGACCAAA
GTTTCTGCATTTAAGGAAAAGAGAATTTGGTAAGCTCAGTAGCTCATCTACCTTAGAGGT
AAGAGAAACATAAAATTTAACAGTACACAGACATAAAAATGCTTAACCATATTTCCTCAA
AAGGAAGAAATAAGTCAGTTCACCTTCTGTTTCTAAACATGATTCAGTATACAACATCAA
TTTTAAGGAACAACTGATATAATGAACGAGCACAAGATGAGAGAATAATGTAAAAGGAGA
AATCAACT
Primers used for PCR CN42 - 8474, show primer details
   
Sequence Name 62-K104191-0022-224-B07C-CN42
GenBank Accession LN510748 [GenBank]
Sequencing primer gene specific primer, CN42: 5'-GGCCAATTTGTAACTAAAATCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K104191-0022-224-B07C-CN42
TCGATGTATATGAAAAAAGAGAAGGAAACTCTTATTCCTTGTTACCAAATCTCCTGCCAA
TGATAGGCCGAACATCATCAGCAACAGCCAAAGTCTCTAAGAAGGGAAGTATAGTAATCA
ACCCATCATCAGTTGGATCATCGTACCAACTTTTGATAGGGATCCCATTATTTATTTGCA
ATCTGTACACCTGAAACAATTAACAAAGAAACAAGTTGGATAATCAAAACGGGTACATGT
ATCATTATACACCAAGAAAGTTTGTAATTTGTAATGTGACAGATATTCTTCAAACTGAAA
CTGATGGTGGGGCCTTTCATCAAATTAATAGTGTTCTCAGTAACAGAGTATATAAAACAT
CATGAAACATCAGACTTTAATGAATAATGTTGAGACCCATGAGCAGATCTTAAGATAAAG
TACCTGCGGACAGTTGTCAATTATATATTCAATTGGATGGACAGTTGTTCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCACGGTTATT
   
Sequence Name 74-K104192-0022-224-B07C-8409
GenBank Accession LN513066 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K104192-0022-224-B07C-8409
TTACATTGAAATATCCTGAACAACTGTCCATCCAATTGAATATATAATTGACAACTGTCC
GCAGGTACTTTATCTTAAGATCTGCTCATGGGTCTCAACATTATTCATTAAAGTCTGATG
TTTCATGATGTTTTATATACTCTGTTACTGAGAACACTATTAATTTGATGAAAGGCCCCA
CCATCAGTTTCAGTTTGAAGAATATCTGTCACATTACAAATTACAAACTTTCTTGGTGTA
TAATGATACATGTACCCGTTTTGATTATCCAACTTGTTTCTTTGTTAATTGTTTCAGGTG
TACAGATTGCAAATAAATAATGGGATCCCTATCAAAAGTTGGTACGATGATCCAACTGAT
GATGGGTTGATTACTATACTTCCCTTCTTAGAGACTTTGGCTGTTGCTGATGATGTTCGG
CCTATCATTGGCAGGAGATTTGGTAACAAGGAATAAGAGTTTCCTTCTCTTTTTTTCTAT
ATACATCGAGTGCAGATCCTGATTTCTCTCCTTTGGATTTTAGTTACAAATTGGC

Last Updated on 06.12.2018 16:11