Sequences from confirmation amplicon


Confirmation sequences for line 225B01 and hit At5g42140 are shown.
Line ID 225B01
Locus At5g42140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16840136
Primers used for PCR UU02 - 8474, show primer details
   
Sequence Name 13-K017255-022-225-B01C-UU02
GenBank Accession LN491822 [GenBank]
Sequencing primer gene specific primer, UU02: 5'-TTTGTTTCAATTTCATCATTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K017255-022-225-B01C-UU02
GCAGTCCTACCAACAGCTCAGTTTGTGCTTCTCGAGATTTTAACATTGCTGATAGTCCCT
ACAATTCAACCAATTTTCCCAGGACTTCTAGAACTGAGAATTCTGTCAGTTCTGAAAGGT
CACATGTGGCATCAGATAGCCCAAATATGTTAGTACGAGGAACTGGATCAGACGCGTTCC
GGGTTAGTGTCTCTAGTGTTCAAAGCAGCTCAAGTCATGGTTCTGCTCCAGATGATTGTG
ATGCTCTAGGTGATGTTTATATATGGGGTGAAGTGCTATGTGAGAATGTTACCAAATTCG
GAGCTGATAAGAATATTGGTTATCTTGGTTCAAGGTCTGATGTTCTGATTCCAAAGCCAT
TGGAGTCCAATGTAGTTTTGGATGTCCATCACATAGCATGTGGTGTTAAGCATGCTGCTC
TAGTCTCTAGGCAAGGTGAGGTTTTTACATGGGGTGAAGCATCCGGAGGACGTCTTGGCC
ATGGAATGGGAAAAGATGTTACTGGACCACAGCTTATTGAGTCTCTACATGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 14-K017255-022-225-B01C-8409
GenBank Accession LN492284 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K017255-022-225-B01C-8409
AGATTTCTCCGACATGAAGCCATTTACATGTAGAGACTCAATAAGCTGTGGTCCAGTAAC
ATCTTTTCCCATTCCATGGCCAAGACGTCCTCCGGATGCTTCACCCCATGTAAAAACCTC
ACCTTGCCTAGAGACTAGAGCAGCATGCTTAACACCACATGCTATGTGATGGACATCCAA
AACTACATTGGACTCCAATGGCTTTGGAATCAGAACATCAGACCTTGAACCAAGATAACC
AATATTCTTATCAGCTCCGAATTTGGTAACATTCTCACATAGCACTTCACCCCATATATA
AACATCACCTAGAGCATCACAATCATCTGGAGCAGAACCATGACTTGAGCTGCTTTGAAC
ACTAGAGACACTAACCCGGAACGCGTCTGATCCAGTTCCTCGTACTAACATATTTGGGCT
ATCTGATGCCACATGTGACCTTTCAGAACTGACAGAATTCTCAGTTCTAGAAGTCCTGGG
AAAATTGGTTGAATTGTAGGGACTATCAGCAATGTTAAAATCTCGAGAAGCACAAACTGA
GCTGTTGGTAGGACTGCTTAATGTCAAGTCTCTACTGTCCTGGAAATGATGAA

Last Updated on 06.12.2018 16:11