Sequences from confirmation amplicon


Confirmation sequences for line 228H05 and hit At4g30996 are shown.
Line ID 228H05
Locus At4g30996 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15102343
Primers used for PCR CP70 - 8474, show primer details
   
Sequence Name 28-K105393-0022-228-H05C-CP70
GenBank Accession LN498844 [GenBank]
Sequencing primer gene specific primer, CP70: 5'-GGATCTTTCAATCAAAGCTGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105393-0022-228-H05C-CP70
AGATCTCGACTTGTAATTGTTTAACAGTTGAATTTTTGTTTTGTTTGCAGGGCTCGCAAA
TCTCTCTATTACAGGTTAGATCTCGATTCTGAGCTTGACTTTGTTTTAGCTGCTATGCTT
GTGTTGTTTCCTTTATTGGTTTTCTATCATGTTGCAATATGGTTTGACAGTTATTGAGAA
GCTTTTATTTTGTAAGATTGGTTTCCCATTTGGTATTTGATACCGGAAAATGAATTCTGT
CTTGTGTTGTGAACAGATTGTGGAAGCGACGATCCAGAGCTTAAACAAGAGATGGAGAAG
CAATTTGTGGACCTTCTGACTGAGGAGCTGAAGCTGCAAGAAGCAGTTGCAGATGAGCAT
AGCCGTCATATGAATGTTACCCTTGCAGAAGCTAAAAGGGTTGCATCTCAGTATCAAAAG
GAGGCTGAGAAGTGCAATGCAGAAGCTGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 12-K105394-0022-228-H05C-8409
GenBank Accession LN491573 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105394-0022-228-H05C-8409
TTCGGACTGAGCCATTTACATTGAATAATCAGCTTCTGCATTGCACTTCTCAGCCTCCTT
TTGATACTGAGATGCAACCCTTTTAGCTTCTGCAAGGGTAACATTCATATGACGGCTATG
CTCATCTGCAACTGCTTCTTGCAGCTTCAGCTCCTCAGTCAGAAGGTCCACAAATTGCTT
CTCCATCTCTTGTTTAAGCTCTGGATCGTCGCTTCCACAATCTGTTCACAACACAAGACA
GAATTCATTTTCCGGTATCAAATACCAAATGGGAAACCAATCTTACAAAATAAAAGCTTC
TCAATAACTGTCAAACCATATTGCAACATGATAGAAAACCAATAAAGGAAACAACACAAG
CATAGCAGCTAAAACAAAGTCAAGCTCAGAATCGAGATCTAACCTGTAATAGAGAGATTT
GCGAGCCCTGCAAACAAAACAAAAATTCAACTTGTTAAACAATATACAAGTCGAGATTCT
TTCTTACTGAAATTAAACCTAAAAGAGACAGCTTTGATTGAAAAGATC

Last Updated on 06.12.2018 16:11