Sequences from confirmation amplicon


Confirmation sequences for line 234D08 and hit At5g46190 are shown.
Line ID 234D08
Locus At5g46190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18723629
Primers used for PCR 07um - 8474, show primer details
   
Sequence Name 63-K117671-0022-234-D08C-07um
GenBank Accession LN511073 [GenBank]
Sequencing primer gene specific primer, 07um: 5'-GATTTTGTTTTATTGCACGGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K117671-0022-234-D08C-07um
GAAGGGGAAAGAATGGTCCTGAAAGACTATAAAATGTCTAGCACGAGAAACCAAAAGTAA
CTGAGTCGAGATGAGATACCGAAATGTTTGATGAGAGATGCAACCATATCTAAACATGAG
CTTGATGGAAGGAGGAAAGCCTGAGAAACCCGATCAAAGAAATCCACTGAGAGGTAGATT
GATGGAACACACTTCCATGGAAAAGGATTAAGGGGCAGAGCAAATTATTCAAGTGGACAT
GATAAAAGCTTGGAACAAGTTCTCTGCGGTACGCTTCTGTTCAGATGTCCCGGAAATGAG
AGCAACGCGACCGCCATGGGAATTTTTGGAATCAGAAATTTCTATCATGGCTCCTGATAT
CTGAGAGAGAGAGAGAGAGAGAGGGCACATACCATGTGAAAATGTTATATAGGAAAGAGG
GAAAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA
   
Sequence Name 15-K117670-0022-234-D08C-8409
GenBank Accession LN493157 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117670-0022-234-D08C-8409
ATTTACATTTTTCCCTCTTTCCTATATAACATTTTCACATGGTATGTGCCCTCTCTCTCT
CTCTCTCTCTCAGATATCAGGAGCCATGATAGAAATTTCTGATTCCAAAAATTCCCATGG
CGGTCGCGTTGCTCTCATTTCCGGGACATCTGAACAGAAGCGTACCGCAGAGAACTTGTT
CCAAGCTTTTATCATGTCCACTTGAATAATTTGCTCTGCCCCTTAATCCTTTTCCATGGA
AGTGTGTTCCATCAATCTACCTCTCAGTGGATTTCTTTGATCGGGTTTCTCAGGCTTTCC
TCCTTCCATCAAGCTCATGTTTAGATATGGTTGCATCTCTCATCAAACATTTCGGTATCT
CATCTCGACTCAGTTACTTTTGGTTTCTCGTGCTAGACATTTTATAGTCTTTCAGGACCA
TTCTTTTCCCCTTTCCTTGCGAGCTGTTCGTACTATAACTACTTATCTCCGTGCAATAAA
ACAAAATC

Last Updated on 06.12.2018 16:11