Sequences from confirmation amplicon


Confirmation sequences for line 246A02 and hit At3g09970 are shown.
Line ID 246A02
Locus At3g09970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3064836
Primers used for PCR PU01 - 8474, show primer details
   
Sequence Name 23-K023619-022-246-A02C-8409
GenBank Accession LN496394 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K023619-022-246-A02C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTTAGATGGTAACATCCATG
CTAATTCCAACAAAGGAGCTATCATAAGTCCACTTGGTTATTCTGTTGCAGGACGATGTT
TGTATAGAAACAGAGGAAGGGCTTAAGCATTGTAAGTTGATCGCCGTACATGCTGGTTTA
GAGAAAGGGAATAACGTAGAAGAACAACTGAAGCTTTTGAGAGCTAAAGACACTAGCATC
TCAAAAATACAACATCTAAGTGGTAGGAAAAACGTTTGGGACATCCCACAGGTAAGGCAT
CGAAAGAATCAAACTTTGTGTTAAGATCTTCCTTAGTTTTTGAAATCTCTTTCTCTGAAA
CATTCTTCAGGAGCTGGAT
   
Sequence Name 24-K023619-022-246-A02C-PU01
GenBank Accession LN496836 [GenBank]
Sequencing primer gene specific primer, PU01: 5'-TATGTTTATCATCCAGCTCCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K023619-022-246-A02C-PU01
GAATGTTTCAGAGAAAGAGATTTCAAAAACTAAGGAAGATCTTAACACAAAGTTTGATTC
TTTCGATGCCTTACCTGTGGGATGTCCCAAACGTTTTTCCTACCACTTAGATGTTGTATT
TTTGAGATGCTAGTGTCTTTAGCTCTCAAAAGCTTCAGTTGTTCTTCTACGTTATTCCCT
TTCTCTAAACCAGCATGTACGGCGATCAACTTACAATGCTTAAGCCCTTCCTCTGTTTCT
ATACAAACATCGTCCTGCAACAGAATAACCAAGTGGACTTATGATAGCTCCTTTGTTGGA
ATTAGCATGGATGTTACCATCTAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11