Sequences from confirmation amplicon


Confirmation sequences for line 255F04 and hit At1g05370 are shown.
Line ID 255F04
Locus At1g05370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1570784
Primers used for PCR 08ve - 8474, show primer details
   
Sequence Name 65-K118051-0022-255-F04C-08ve
GenBank Accession LN511488 [GenBank]
Sequencing primer gene specific primer, 08ve: 5'-CAGAAGCTACATACGCAGAAACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K118051-0022-255-F04C-08ve
CACGTTTGGAGGTATTTACATTAGAGGTTGCTATCTCAACCACGTCCAGAAACGTCGAAC
AATTTGTCATCCTTTTCGATGCTAGTAAGTTCTCTCTTATGTTCAATTTATCATTTTATA
TCACTAGGAAAAAGGTTTTCAAAATAAAGGGGGAAAAGGTTAAAAATTTCAAAATATGGC
AACTATTTTTATTTTGTCTTCTTATCTTTCAATGGAAATATTGTCACCGTTTACGAATAA
ACATAAATGTGATTTTTACAAAAAGAGAAGTTGGTAAAAATTGGGGACAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 29-K118050-0022-255-F04C-8409
GenBank Accession LN499469 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118050-0022-255-F04C-8409
TTGAAATATCCTGTCCCCAATTTTTACCAACTTCTCTTTTTGTAAAAATCACATTTATGT
TTATTCGTAAACGGTGACAATATTTCCATTGAAAGATAAGAAGACAAAATAAAAATAGTT
GCCATATTTTGAAATTTTTAACCTTTTCCCCCTTTATTTTGAAAACCTTTTTCCTAGTGA
TATAAAATGATAAATTGAACATAAGAGAGAACTTACTAGCATCGAAAAGGATGACAAATT
GTTCGACGTTTCTGGACATGGTTGAGATAGCAACCTCTAATGTAAATACCACCAAACGTG
TCAACCTACGAAAATAAACGAATATATAAATAAACTTTGTGAATAATGTTTTTGATGTAT
TAATATGTATGAGTAATTTAACTTACTGTTTCTGCGTAGTTAGGCTTCTG

Last Updated on 06.12.2018 16:11