Sequences from confirmation amplicon


Confirmation sequences for line 258B05 and hit At1g07650 are shown.
Line ID 258B05
Locus At1g07650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2365384
Primers used for PCR MK64 - 8474, show primer details
   
Sequence Name 24-K101424-0022-258-B05C-8409
GenBank Accession LN496931 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K101424-0022-258-B05C-8409
GAGCCATTTACATTGATCCATGTAGGTGGATGTAAATTCACATGTGAAGTAAGATTAAAA
AGCTTTAAGTTTGATTTGTCTCTTTGTGACGACAAGTTTATACTTGCCAGGCTGGTCGTT
GACCCGTTGACCAAAGTAAACGATTCAATAGATAATAGCGCGTTTACTTTCGCTCTATTC
CATAACTAGACTTTATATAAATCGCCAGATAAAAAACTATGATTTACATAATTCAAAAAA
CATTTAGTGTTAGTAAAAATTAGTTATATATTTGTGTCTCTTTAGACTGTTTGATCATTT
TTTAAAATCATAATTCTTTTTAAAAAATTGTCATCAAAATCGTAACAAGTTTATGATCCA
AAATAATATAAGAACAACCATGATCTGTAATTCTGTATTATCAAACAGTAACACTACTGT
AAATTTGTTTCATTTTGAGATGATGAAAAATCAGAAATTATTTTTCTAGTACAAATTTCA
GTTTTAAGAAGAATAAAACAAATTTCATACCGATGCGACAATTACTTTACCAGTCCATTA
AATATTGTTTTAATTTGTTTCCCTAGCTTATGCTTAAGTATATTACAATATTTGTCCCCG
TTGTTCTTGAGAAGTTTATCTTCCA
   
Sequence Name 64-K101427-0022-258-B05C-MK64
GenBank Accession LN511162 [GenBank]
Sequencing primer gene specific primer, MK64: 5'-AAGTTTTGGGGTGGAAGATAAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K101427-0022-258-B05C-MK64
TATATACTTAGCAAAGCTAGGGAAAAAATTAAAACAATATTTAATGGTTTGGTAAAGTAA
TTGTCGCATCGGTATGAAATTTGTTCTATTCTTCTTAAAACTGAAATTTGTACTAGAAAA
ATAATTTCTGATTTTTCATCATCTCAAAATGAAACAAATTTACAGTAGTGTTACTGTTTG
ATAATACAGAATTACAGATCATGGTTGTTCTTATATTATTTTGGATCATAAACTTGTTAC
GATTTTGATGACAATTTTTTAAAAAGAATTATGATTTTAAAAAATGATCAAACAGTCTAA
AGAGACACAAATATATAACTAATTTTTACTAACACTAAATGTTTTTTGAATTATGTAAAT
CATAGTTTTTTATCTGGCGATTTATATNATGTCTAGTTATGGAATAGAGCGAAAGTAAAC
GCGCTATTATCTATGGAATCGTTTACTTTGGTCAACGGGTCAACGACCAGCCCGGCAAGT
ATAAACTTGTCGT

Last Updated on 06.12.2018 16:11