Sequences from confirmation amplicon


Confirmation sequences for line 265A02 and hit At3g27580 are shown.
Line ID 265A02
Locus At3g27580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10219491
Primers used for PCR DL38 - 8474, show primer details
   
Sequence Name 03-K018490-022-265-A02C-DL38
GenBank Accession LN487279 [GenBank]
Sequencing primer gene specific primer, DL38: 5'-CAACCCAACGCTTATCGTTATTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K018490-022-265-A02C-DL38
TTGTAAGCTGCATTACTCAGACCACTTGAGCTACTCTCATCACTAACATCACTCCCTATA
CTTCCTCTGTATATCATTACACCATTCTTCTCCAAATCAATACCACCTATACCAATAGTG
CTTATGCTTGCACATTCACTGAATTCTGTGTTGGTAAAGCTTTGTTTTGCTTCTGTGTAA
AACGTGTTGGTTGGGCTAGAGTAAAACCTTGGCTCTGATTGTTTCTTCTTACGTATAATC
GAAATTGGTGTCTCGGATTCAGCTGAAACAGAGGACACAGAACTTTCTATAACTTCAGGG
GAAGTTGGTTCTGGTTCTGGGATTAATAACGTCTTCATTGATTTCTTGGTGCCTCTTGAA
TCGATTGAGAAAGATGTAATCGAACCTTTGGATTTAGATTGAAAGTTTCTGGATTTGGAT
GATGAACCAACGTAAACGACTGATGAAGAAGAATCCATTGAAGGTATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATTNGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
   
Sequence Name 04-K018490-022-265-A02C-8409
GenBank Accession LN487722 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K018490-022-265-A02C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATACCTTCAATGGATTCTT
CTTCATCAGTCGTTTACGTTGGTTCATCATCCAAATCCAGAAACTTTCAATCTAAATCCA
AAGGTTCGATTACATCTTTCTCAATCGATTCAAGAGGCACCAAGAAATCAATGAAGACGT
TATTAATCCCAGAACCAGAACCAACTTCCCCTGAAGTTATAGAAAGTTCTGTGTCCTCTG
TTTCAGCTGAATCCGAGACACCAATTTCGATTATACGTAAGAAGAAACAATCAGAGCCAA
GGTTTTACTCTAGCCCAACCAACACGTTTTACACAGAAGCAAAACAAAGCTTTACCAACA
CAGAATTCAGTGAATGTGCAAGCATAAGCACTATTGGTATAGGTGGTATTGATTTGGAGA
AGAATGGTGTAATGATATACAGAGGAAGTATAGGGAGTGATGTTAGTGATGAGAGTAGCT
CAAGTGGTCTGAGTAATGCAGCTTACAAGCCACATAGAGATAATAACG

Last Updated on 06.12.2018 16:11