Sequences from confirmation amplicon


Confirmation sequences for line 270A05 and hit At3g29410 are shown.
Line ID 270A05
Locus At3g29410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11303674
Primers used for PCR 09pk - 8474, show primer details
   
Sequence Name 55-K115187-0022-270-A05C-09pk
GenBank Accession LN508896 [GenBank]
Sequencing primer gene specific primer, 09pk: 5'-AAGGAAAGTTTAGTTGGGGATGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K115187-0022-270-A05C-09pk
AGCGAGCATCTGGAGCACATCTGAAGATATAATGGATGAAGCCTTAAGTTTTGCGCGGTA
TCACTTGGAACCGTTGGCAGGCACCGAAACAAGCTCTAATCTTTTTAAGCATGTAGAAAA
CGTTTTGTATAGAGCTCGATATCATAGCATAGAGATACTAGTAGCACGACAGTACATCTC
TTTCTATGATCAAGAAGAAGACCAAGATGAGACGCTCCTTAGGTTTTCAAAGCTCAATTT
CAATTTTTGCCAGATGCATTACGTCAAAGAGCTCAAAATTGTCACTAGGTAAGTATATTG
TATATATATAACATCTTTCGTCGTCAATATTCTTTTTTATGTAACCTAATTTATGGTTCT
AGATGGTGGAAGGAGCTAGGTATCGCATCTAAGCTACCGTACTCCATAAGGGAGCGAAAT
GTGGAGACTTATCTTGGGGGCTTGGGAGTGCTTTTCGAGCCGCGATATTCACTTGCAAGA
ATTTTCTTAGCTAAACTAACACTGATCATGACTGTTGTGGATGATACATGTGATGCATAT
GCCACTCTTCCTGAAGTTCAGAGTCTTCATGATGCTTTTCATAGGTATGTATGTAGAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 19-K115186-0022-270-A05C-8409
GenBank Accession LN494885 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115186-0022-270-A05C-8409
TTCTCATACATACCTATGAAAAGATCATGAAGACTCTGAACTTCAGGAAGAGTGGCATAT
GCATCACATGTATCATCCACAACAGTCATGATCAGTGTTAGTTTAGCTAAGAAAATTCTT
GCAAGTGAATATCGCGGCTCGAAAAGCACTCCCAAGCCCCCAAGATAAGTCTCCACATTT
CGCTCCCTTATGGAGTACGGTAGCTTAGATGCGATACCTAGCTCCTTCCACCATCTAGAA
CCATAAATTAGGTTACATAAAAAAGAATATTGACGACGAAAGATGTTATATATATACAAT
ATACTTACCTAGTGACAATTTTGAGCTCTTTGACGTAATGCATCTGGCAAAAATTGAAAT
TGAGCTTTGAAAACCTAAGGAGCGTCTCATCTTGGTCTTCTTCTTGATCATAGAAAGAGA
TGTACTGTCGTGCTACTAGTATCTCTATGCTATGATATCGAGCTCTATACAAAACGTTTT
CTACATGCTTAAAAAGATTAGAGCTTGTTTCGGTGCCTGCCAACGGTTCCAAGTGATACC
GCGCAAAACTTAAGGCTTCATCCATTATATCTTCAGATGGTGCTCCTAGATGCGCGGCTT
CGTACAGCTGTAGCAGCCCTCTAACATCCCCAACTAACTTTTCC

Last Updated on 06.12.2018 16:11