Sequences from confirmation amplicon


Confirmation sequences for line 270E05 and hit At1g02970 are shown.
Line ID 270E05
Locus At1g02970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:675284
Primers used for PCR AS09 - 8474, show primer details
   
Sequence Name 15-K018334-022-270-E05C-AS09
GenBank Accession LN492756 [GenBank]
Sequencing primer gene specific primer, AS09: 5'-TCAATAAGGCTTGGTTTCTTCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K018334-022-270-E05C-AS09
TACTAAATATACTGTTATGTAGGTTTTCTCCCTGTAAGTACAAGTGGGGATGGCTTGTCA
AGATATCTCACGGACTTTCATGAAATCCGGGTAAGCGAAAGGGTCTTTTAGCTTGGAATT
GTGATCATGACGGAATTATGTTGCTTCTTCTTACGTATTTTGGTTTGGTTTCTCCAGCAA
ATTGGTGCTGGACATTTCAGTCGGGTATTTAAGGTGTTGAAGAGAATGGACGGTTGCCTA
TATGCTGTGAAACACAGCACAAGAAAGTTGTATCTAGATTCAGAGAGGTATAATTTTCTC
TAGTTTTCTTAGTAAGAGAAAGACACATATCTGCTGAAAATAGTTCATCTTGTTTGGATC
GTTTTTGTTCAAGTAATCACAGCTGAATTTTTGCAGACGTAAAGCTATGATGGAAGTGCA
AGCTCTTGCTGCTCTTGGTCAGTACAGTCCCTTCTTTGGCCTTCTTGTTCTCAAACTATT
CTGTTATTCCCTGTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCANAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACNAATTACG
   
Sequence Name 16-K018334-022-270-E05C-8409
GenBank Accession LN493225 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018334-022-270-E05C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCACAGGGAATAACAGAATA
GTTTGAGAACAAGAAGGCCAAAGAAGGGACTGTACTGACCAAGAGCAGCAAGAGCTTGCA
CTTCCATCATAGCTTTACGTCTGCAAAAATTCAGCTGTGATTACTTGAACAAAAACGATC
CAAACAAGATGAACTATTTTCAGCAGATATGTGTCTTTCTCTTACTAAGAAAACTAGAGA
AAATTATACCTCTCTGAATCTAGATACAACTTTCTTGTGCTGTGTTTCACAGCATATAGG
CAACCGTCCATTCTCTTCAACACCTTAAATACCCGACTGAAATGTCCAGCACCAATTTGC
TGGAGAAACCAAACCAAAATACGTAAGAAGAAGCAACATAATTCCGTCATGATCACAATT
CCAAGCTAAAAGACCCTTTCGCTTACCCGGATTTCATGAAAGTCCGTGAGATATCTTGAC
AAGCCATCCCCACTTGTACTTACAGGGAGAAAACCTACATAACAGTATATTTAGTACAGT
TTTACTGAAGAA

Last Updated on 06.12.2018 16:11