Sequences from confirmation amplicon


Confirmation sequences for line 271A03 and hit At1g19500 are shown.
Line ID 271A03
Locus At1g19500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6754973
Primers used for PCR KR46 - 8474, show primer details
   
Sequence Name 74-K100822-0022-271-A03C-8409
GenBank Accession LN513025 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K100822-0022-271-A03C-8409
ATTTACAATTGAATATATCCTGAAGAAAGATAACAAATCATATATTAAGGAGGGATTATG
GAAATTGATTATTAAAACAAAATTGGTGCTTACAGTTGATAAAATCATAAAAATTTAATG
TTTAACCTAGGCAAATTACTATTAAATAGATACAGAAATCATGGTATCATCAAATAGATT
TATAACGCCATGGGTTTCTGTGGTGTTTTGTATGAATTATCATGTTACTATATATCGAAT
TTAGATTCAGTTTTTGTGTTGTGGTGTTGTATCGTAGGAACTAAAAATTAAAGTTACGGG
ATAGTAAAAGTGATTTTTGTACCAAAAAGATTAAAAATATTCAAAAGAATCGTAGACATA
CTTGGCTTTATCACCGAA
   
Sequence Name 74-K100834-0022-271-A03C-KR46
GenBank Accession LN513027 [GenBank]
Sequencing primer gene specific primer, KR46: 5'-GTTCGGTGATAAAGCCAAGTATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K100834-0022-271-A03C-KR46
TTTATCTTTTTGGACAAAAATCACTTTTACTATCCCGTAACTTTAATTTTTAGTTCCTAC
GATACAACACCACAACACAAAAACTGAATCTAAATTCGATATATAGTAACATGATAATTC
ATACAAAACACCACAGAAACCCATGGCGTTATAAATCTATTTGATGATACCATGATTTCT
GTATCTATTTAATAGTAATTTGCCTAGGTTAAACATTAAATTTTTATGATTTTATCAACT
GTAAGCACCAATTTTGTTTTAATAATCAATTTCCATAATCCCTCCTTAATATATGATTTG
TTATCTTTCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11