Sequences from confirmation amplicon


Confirmation sequences for line 271F04 and hit At1g33080 are shown.
Line ID 271F04
Locus At1g33080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11985826
Primers used for PCR 19hw - 8474, show primer details
   
Sequence Name 29-K112810-0022-271-F04C-19hw
GenBank Accession LN499365 [GenBank]
Sequencing primer gene specific primer, 19hw: 5'-TAGTTTGCATTTTTGTTTTGTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K112810-0022-271-F04C-19hw
CATTATTGGATTTGGTTTGGTTATAAAGATGTGTCTATTCAACACCAACTTTTGGTAAAA
TATTTGTAGAGGCCATACCTCGCTGTGATATATATTTCGACTATCTCATCCCATTTTTTG
GGTGATTTGTTCAAAACTAGCTATGTCTTTTTCGGTATGCAATGTATAAATGTAAATGTA
ATTTTGACAAATTTTAAATTTTATTTAAAATTTATTTATGAAAAGTATTCATTTTGCATA
TAAAATACCAATTTGGCAAATTAAAGAATCTTAAAAAGAGGTGTAGGCCGGCTTTTCATT
TCCTCCGTACGTAAATAAACAACCCACCAAACAAAAATCTCTCCTCGCTCCATGCCTTCT
CTACTCTCTCTTCATTCTCTCCTAAGCTATGGCGAGAAGAGAAGGAGAAGTGACGGAGAC
CCTTCTGAAAAAGTCGACGGAGAATCGCGGAGAAGATCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 04-K112809-0022-271-F04C-8409
GenBank Accession LN487990 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K112809-0022-271-F04C-8409
ATCCTGATCTTCTCCGCGATTCTCCGTCGACTTTTTCAGAAGGGTCTCCGTCACTTCTCC
TTCTCTTCTCGCCATAGCTTAGGAGAGAATGAAGAGAGAGTAGAGAAGGCATGGAGCGAG
GAGAGATTTTTGTTTGGTGGGTTGTTTATTTACGTACGGAGGAAATGAAAAGCCGGCCTA
CACCTCTTTTTAAGATTCTTTAATTTGCCAAATTGGTATTTTATATGCAAAATGAATACT
TTTCATAAATAAATTTTAAATAAAATTTAAAATTTGTCAAAATTACATTTACATTTATAC
ATTGCATACCGAAAAAGACATAGCTAGTTTTGAACAAATCACCCAAAAAATGGGATGAGA
TAGTCGAAATATATATCACAGCGAGGTATGGCCTCTACAAATATTTTACCAAAAGTTGGT
GTTTGAATAGACACATCTTTTATAACCAAACCAAATCCAATTAATTGTTGTAAGAGTTTA
AGGTTTTTTGAAACGAACAAAACAAAATGGCAAACT

Last Updated on 06.12.2018 16:11