Sequences from confirmation amplicon


Confirmation sequences for line 273B07 and hit At3g23820 are shown.
Line ID 273B07
Locus At3g23820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8604973
Primers used for PCR MP09 - 8474, show primer details
   
Sequence Name 33-K016849-022-273-B07C-8409
GenBank Accession LN500788 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K016849-022-273-B07C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGTAAATAATAACCAACGTACCACTTGACGAA
CTTCCTCAGCCCCGCCGCGAGATCCGTCGTCGGTTTGTACCCGAAATCTTTATACGCTAA
ACTCACATTAGCATGCGTGTAAGGCACGTCACCGTTTCTTGGCATTTTGATAAGATGCTT
CTTAGCTTTTGTCCCTAACAATCCTTCCAAGATCGAGACCAATCTCCCAACCGGAACCGG
GGACGTGTTTCCTAGATTGTAAACACGTAGCTGAGCTTGTCCTCGCTTCTTTCCGCCGCT
TCCGGTGCTTTTCTCAGCCGTGTCTAACGCACCGACACAACCTTTAACGATGTCATCGAT
GTAGGTGAAGTCACGCGCCACTTCTTGATTGTCCTGAGTTCTGANATGTC
   
Sequence Name 34-K016849-022-273-B07C-MP09
GenBank Accession LN501213 [GenBank]
Sequencing primer gene specific primer, MP09: 5'-CGACATTTACAGAACTCAGGACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K016849-022-273-B07C-mp09
TGGCGCGTGACTTCACCTACATCGATGACATCGTTAAAGGTTGTGTCGGTGCGTTAGACA
CGGCTGAGAAAAGCACCGGAAGCGGCGGAAAGAAGCGAGGACAAGCTCAGCTACGTGTTT
ACAATCTAGGAAACACGTCCCCGGTTCCGGTTGGGAGATTGGTCTCGATCTTGGAAGGAT
TGTTAGGGACAAAAGCTAAGAAGCATCTTATCAAAATGCCAAGAAACGGTGACGTGCCTT
ACACGCATGCTAATGTGAGTTTAGCGTATAAAGATTTCGGGTACAAACCGACGACGGATC
TCGCGGCGG

Last Updated on 06.12.2018 16:11