Sequences from confirmation amplicon


Confirmation sequences for line 274F09 and hit At3g56870 are shown.
Line ID 274F09
Locus At3g56870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21054135
Primers used for PCR CK47 - 8474, show primer details
   
Sequence Name 40-K018157-022-274-F09C-CK47
GenBank Accession LN503547 [GenBank]
Sequencing primer gene specific primer, CK47: 5'-GAAATAATGCTTGATTCGTCCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K018157-022-274-F09C-CK47
GCTGGTTCCATACTGCAGTGCAGAGCCAAATTTTCCCGGAGTGGTAGCCATTGAGGAAGC
TTTTGAAGATGCATCAGAAGAGTTTAGCGATGCTGATTTTCAGAATAAGCAAATTGTTTT
GTACTCTAGTGTGTCTAGAGAGGAGATGGAATTGGATGTTAATCCACAACATTCAGAATA
TGAGAACATTGGTTGTGTAAAGAACTTCATTTCTGCTTACACAAGCTCTGGGTGCGAAGA
AGAAGATAAAGAGGATGAGGAAAGCAATGATATAAAGGCTAACTTAGATATGAGTGTAAC
TGGTTTGGAGATTGTGAAGATAGAAGCTCCAGAAATACTTGCTATTGATTACCCCGGTTG
TTTGTCCATCATCAATTTTTGTGCAGAAGATTCCGAGATTGTGAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATTGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
   
Sequence Name 41-K018157-022-274-F09C-8409
GenBank Accession LN503903 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K018157-022-274-F09C-8409
TAGATTTCCCGGACATGAAGCCATTCACAATCTCGGAATCTTCTGCACAAAAATTGATGA
TGGACAAACAACCGGGGTAATCAATAGCAAGTATTTCTGGAGCTTCTATCTTCACAATCT
CCAAACCAGTTACACTCATATCTAAGTTAGCCTTTATATCATTGCTTTCCTCATCCTCTT
TATCTTCTTCTTCGCACCCAGAGCTTGTGTAAGCAGAAATGAAGTTCTTTACACAACCAA
TGTTCTCATATTCTGAATGTTGTGGATTAACATCCAATTCCATCTCCTCTCTAGACACAC
TAGAGTACAAAACAATTTGCTTATTCTGAAAATCAGCATCGCTAAACTCTTCTGATGCAT
CTTCAAAAGCTTCCTCAATGGCTACCACTCCGGGAAAATTTGGCTCTGCACTGCAGTATG
GAACCAGCCTCATGTCACTGGACGAATC

Last Updated on 06.12.2018 16:11