Sequences from confirmation amplicon


Confirmation sequences for line 278D11 and hit At1g52290 are shown.
Line ID 278D11
Locus At1g52290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19471945
Primers used for PCR DL77 - 8474, show primer details
   
Sequence Name 44-K018602-022-278-D11C-DL77
GenBank Accession LN505032 [GenBank]
Sequencing primer gene specific primer, DL77: 5'-CAAATCATCCTCATCATCTTCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K018602-022-278-D11C-DL77
TCTACCGACACTATCCCTTCCCTCTCATCTCCACCGGCGCCGGAGTTTCCTTCCACCACA
CCAGACACGGCGACGTCACCAGCTCCGTCTCAGCCATCCATCATCGGACCTTCTTCGTTG
GCTCCGTTTCCGGAGACAACAACAAACATTGATGGAGGCTCGAGAAATGTAGCATTAACT
GGACTTATTACTGGAGTAGTTCTCGGAGCTACGTTTGTCCTACTCGGAGTTTGTATCTTT
GTCTGTTTCTATAAAAGAAAGAAGAGGAAGTTAAAGAAGAAGAAGAAAGAAGATATCGAA
GCTTCTATTAACAGAGACTCACTAGATCCCAAAGGTACTTTATTTAGGGTTTCTTTATTT
CCAAAGACTCAATCTTTTTTTAATGATTATTCAATTCCTCTGTTTTTGTAGATGAGAAAC
AGATACCTTGTTTTATCAGATTATTCAATTTAAACAGATACCTTCAGCAATAATCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 45-K018602-022-278-D11C-8409
GenBank Accession LN505370 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K018602-022-278-D11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGATTATTGCTGAAGGTATCTGTTTAAAT
TGAATAATCTGATAAAACAAGGTATCTGTTTCTCATCTACAAAAACAGAGGAATTGAATA
ATCATTAAAAAAAGATTGAGTCTTTGGAAATAAAGAAACCCTAAATAAAGTACCTTTGGG
ATCTAGTGAGTCTCTGTTAATAGAAGCTTCGATATCTTCTTTCTTCTTCTTCTTTAACTT
CCTCTTCTTTCTTTTATAGAAACAGACAAAGATACAAACTCCGAGTAGGACAAACGTAGC
TCCGAGAACTACTCCAGTAATAAGTCCAGTTAATGCTACATTTCTCGAGCCTCCATCAAT
GTTTGTTGTTGTCTCCGGAAACGGAGCCAACGAAGAAGGTCCGATGATGGATGGCTGAGA
CGGAGCTGGTGACGTCGCCGTGTCTGGTGTGGTGGAAGGAAACTCCGGCGCCGGTGGAGA
TGAGAGGGAAGGGATAGTGTCGGTAGACATTAGATCAGAAGATGATG

Last Updated on 06.12.2018 16:11