Sequences from confirmation amplicon


Confirmation sequences for line 292E08 and hit At3g51240 are shown.
Line ID 292E08
Locus At3g51240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19025278
Primers used for PCR MM70 - 8474, show primer details
   
Sequence Name 81-K021647-022-292-E08C-8409
GenBank Accession LN514003 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K021647-022-292-E08C-8409
GCCATTTACAATGAGTTGAGGTACTCATTGCGACCTCTTCGTTCGTCAGTCATCACAAGC
TTTGAAAGATTTTCAGCTACCACTCTCTCCTTTATATATTCATTACACATCTCTTCTTTC
TATATCTCTCTTAATTTAGTCTTTTGTCTTCGTAATTACAATGGCTCCAGGAACTTTGAC
TGAGCTAGCCGGAGAGTCTAAGCTCAACTCTAAATTTGTCAGGGACGAAGATGAACGGCC
CAAAGTCGCTTACAATGTGTTTAGCGACGAAATCCCGGTGATCTCTCTCGCCGGTATCGA
TGACGTCGATGGAAAAAGAGGAGAGATCTGCCGTCAGATCGTTGAGGCTTGTGAGAATTG
GGGCATCTTCCAAGTGGTCGATCACGGCGTCGATACTAACTTAGTGGCGGATATGACTCG
TCTCGCTCGTGACTTCTTTGCTTTACCTCCGGAAGACAAGCTCCGTTTCGACATGTCCGG
TGGTAAAAAAGGAGGATTCATCGT
   
Sequence Name 82-K021647-022-292-E08C-MM70
GenBank Accession LN514126 [GenBank]
Sequencing primer gene specific primer, MM70: 5'-GAGGTGACTAGAGACGATGAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K021647-022-292-E08C-MM70
CTTGTCTTCCGGAGGTAAAGCAAAGAAGTCACGAGCGAGACGAGTCATATCCGCCACTAA
GTTAGTATCGACGCCGTGATCGACCACTTGGAAGATGCCCCAATTCTCACAAGCCTCAAC
GATCTGACGGCAGATCTCTCCTCTTTTTCCATCGACGTCATCGATACCGGCGAGAGAGAT
CACCGGGATTTCGTCGCTAAACACATTGTAAGCGACTTTGGGCCGTTCATCTTCGTCCCT
GACAAATTTAGAGTTGAGCTTAGACTCTCCGGCTAGCTCAGTCAAAGTTCCTGGAGCCAT
TGTAATTACGAAGACAAAAGACTAAATTAAGAGAGATATAGAAAGAAGAGATGTGTAATG
AATATATAAAGGAGAGAGTGGTAGCTGAAAATCTTTCAAAGCTTGTGATGACTGACGAAC
GAAGAGGTCGCAATGAGTACCTCAACTCATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11