Sequences from confirmation amplicon


Confirmation sequences for line 297C08 and hit At5g21100 are shown.
Line ID 297C08
Locus At5g21100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7169887
Primers used for PCR HV40 - 8474, show primer details
   
Sequence Name 33-K033099-022-297-C08C-HV40
GenBank Accession LN500894 [GenBank]
Sequencing primer gene specific primer, HV40: 5'-CAGCGTATTTTAACAAAGGAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K033099-022-297-C08C-HV40
ATGATCAGTGTGCACCTCAACTCTTCGAGTCGAACCCAATAGAGTGTACCGTCTTCGAAT
CGCTAGCACAACTGCTCTTGCTTCCCTCAACTTGGCTGTTCAAGTAAGTTTCTTTCTTTT
CAACTCACACGTGTACTTTAAAAGCTCACACGTGTGGTTTGATTGCGCACGTGACAGGGA
CACCAGCTGGTGGTTGTTGAAGCTGACGGCAACTACGTCGCACCGTTCACCGTCAACGAC
ATTGACGTTTATTCCGGCGAAACTTATTCTGTTCTCCTTAAAACCAACGCACTTCCATCA
AAGAAGTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 81-K033099-022-297-C08C-8409
GenBank Accession LN514012 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K033099-022-297-C08C-8409
TTTACATTGAATATATCCTGTACTTCTTTGATGGAAGTGCGTTGGTTTTAAGGAGAACAG
AATAAGTTTCGCCGGAATAAACGTCAATGTCGTTGACGGTGAACGGTGCGACGTAGTTGC
CGTCAGCTTCAACAACCACCAGCTGGTGTCCCTGTCACGTGCGCAATCAAACCACACGTG
TGAGCTTTTAAAGTACACGTGTGAGTTGAAAAGAAAGAAACTTACTTGAACAGCCAAGTT
GAGGGAAGCAAGAGCAGTTGTGCTAGCGATTCGAAGACGGTACACTCTATTGGGTTCGAC
TCGAAGAGTTTGAGGTGCACACTGATCATTTTCTTTAAACGTGCATACATCTTTCTCTCC
TCCTTTGTAAAAATACGCTG

Last Updated on 06.12.2018 16:11