Sequences from confirmation amplicon


Confirmation sequences for line 298B10 and hit At2g38630 are shown.
Line ID 298B10
Locus At2g38630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16155488
Primers used for PCR 39qa - 8474, show primer details
   
Sequence Name 50-K116249-0022-298-B10C-39qa
GenBank Accession LN507287 [GenBank]
Sequencing primer gene specific primer, 39qa: 5'-GTGTCTGTGCATCTTTTAGCAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K116249-0022-298-B10C-39qa
CTGTAATTTGATGGGTTTGTTTGTTGTAATTGCAGAGACGAATAAAAAGGTTTGCTTTTT
AATGTTAGTCCTGATGAAGTCATTCGGAGTTTGTTCTACAACAAGAACAATGATTCTCTC
ATTACTGTTTCTGTTTACGCCTCTGACAATTACAGCTCTTTGAAATGCAGATCCACTAGA
ATCGAGTATGTCTACTTACTTACTCTTGTGATGTTGTTGTCTTGTTAAGTTAGTTCTCAG
ACTTGTGAAAGTAATGTTTTTTGTTTTGTTAGATATATTCTGAGGGGTCAAGCAGACGCA
GGATTTCCTCTTTTTGAGTCTGAGTCACTAAAGTGGCCAGGTTTTGTTGAGTAAATGGCT
TCAATGTAGATTTCTTTTTCATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGC
   
Sequence Name 26-K116248-0022-298-B10C-8409
GenBank Accession LN498079 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116248-0022-298-B10C-8409
GAATCTACATTGAAGCCATTTACTCAACAAAACCTGGCCACTTTAGTGACTCAGACTCAA
AAAGAGGAAATCCTGCGTCTGCTTGACCCCTCAGAATATATCTAACAAAACAAAAAACAT
TACTTTCACAAGTCTGAGAACTAACTTAACAAGACAACAACATCACAAGAGTAAGTAAGT
AGACATACTCGATTCTAGTGGATCTGCATTTCAAAGAGCTGTAATTGTCAGAGGCGTAAA
CAGAAACAGTAATGAGAGAATCATTGTTCTTGTTGTAGAACAAACTCCGAATGACTTCAT
CAGGACTAACATTTAAAAAGCAAACCTTTTTATTCGTCTCTGCAATTACATACAAACAAA
CCCATCAAATTACAGTGTCACACAAAGCAAAAAAGCTTAAAAAGTTAGTACCTCTGCTAA
AAGATGAACAGACAC

Last Updated on 06.12.2018 16:11