Sequences from confirmation amplicon


Confirmation sequences for line 298E04 and hit At4g38290 are shown.
Line ID 298E04
Locus At4g38290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17943315
Primers used for PCR FV80 - 8474, show primer details
   
Sequence Name 17-K100895-0022-298-E04C-8409
GenBank Accession LN493776 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K100895-0022-298-E04C-8409
GAGCCATTTACAATTGAATATATCCTGTACTTGAGACAGCCTAATACTACCAAGTCCATT
GCATTTTCTGTTATCCCCCTGTTCTATATATAGATTTGAAGAGATGAGATTTTTAAATTG
CTACAAAAGAGAGAGTCTACAATTTCGCAATTATCAACAAAATTATAGAGAGAGAGAGAG
AGGCTTTTGACATTTTTGGTCTGTAAGATATGTAAATAGTAGAAAGGGAGGGGGAGTGAG
TATTTTGCAAAAGAGTGTTTGAAACTTGAAAAGTGAAGTAAAATAAGGACTAGGAGGGGA
TGCATGCTTATACCATTATGACATGTTCCTTTTGGAATGGGATTCTCTGGCTTAGCTCCG
ATTCTTCACAAGCTGATAATCTTTTGGGACCAACCTGAAGCCCTTCACACGACAGGTTAT
GAGATTTTGATGGGTTTGCTTTATGGGTTAGGAGCTCTGGTTTATGCA
   
Sequence Name 17-K100903-0022-298-E04C-FV80
GenBank Accession LN493777 [GenBank]
Sequencing primer gene specific primer, FV80: 5'-GATCCTAGTTGCATAAACCAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K100903-0022-298-E04C-FV80
ACCATCAAATCTATAACCTGTCGTGTGAAGGGCTTCAGGTTGGTCCCAAAAGATTATCAG
CTTGTGAAGAATCGGAGCTAAGCCAGAGAATCCCATTCCAAAAGGAACATGTCATAATGG
TATAAGCATGCATCCCCTCCTAGTCCTTATTTTACTTCACTTTTCAAGTTTCAAACACTC
TTTTGCAAAATACTCACTCCCCCTCCCTTTCTACTATTTACATATCTTACAGACCAAAAA
TGTCAAAAGCCTCTCTCTCTCTCTCTATAATTTTGTTGATAATTGCGAAATTGTAGACTC
TCTCTTTTGTAGCAATTTAAAAATCTCATCTCTTCAAATCTATATATAGAACAGGGGGAT
AACAGAAAATGCAATGGACTTGGTAGTATTAGGCTGTCTCAAGTACAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT

Last Updated on 06.12.2018 16:11