Sequences from confirmation amplicon


Confirmation sequences for line 300A12 and hit At5g06110 are shown.
Line ID 300A12
Locus At5g06110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1842223
Primers used for PCR PF55 - 8474, show primer details
   
Sequence Name 41-K103731-0022-300-A12C-PF55
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, PF55: 5'-ATGACCATTGGCTTCTATATGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K103731-0022-300-A12C-PF55
AACAGCTGGCTCACCATCTGCCTCCTGCTGCCCCTCGTCCATCAGCTTTGCCTCATTCTT
CTGCTTCTCCTTACCCTTTTGCACTTTCATTCCTTTCTGAAATCCAATGTCTTTTTGAAA
TCCATTGCCCACATGAGTCCTAATGCCAGCATAATCCTTCTTTCTAGCATTCTCACTTTT
TCTTGCATTCTCACTCTTTCTCGAAATCTTTTCCTAAGTGGAAATCTGGTCCGAATTGGA
TGTGAAGGGCAGCTGGTCCGGGAATTCCACCTGGATCAGCAATGAAAATTATGGCCAATT
TGG
   
Sequence Name 57-K103732-0022-300-A12C-8409
GenBank Accession LN509394 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K103732-0022-300-A12C-8409
TCGGACTGAGCCTTTACATTGATTTGGACATGATATCAATTGAGGAAAAGAGGTGGATGG
AGAGAGAGAATGCAAGAAAAACTCAGAAGGCTAGAAAGGAGGAATATGCTCGGATTAGGA
CTCTTGTGGACAACGCATATAAAAAAGACATAAGAATACAGAAAAGAAAGGATGACGAAA
AGGCTAAGAAGCTGCAGAAGAAAGAAGCAAAAGTTATGGCCAAGAGGCAGCAGGAAGAAG
CTGCTGCTGCAGCTATTGAAGAAGAGAAAAGGCGAAAAGAAGAAGAAGCAAAACGTGCGG
CAGAGGCTGCTCAGCTGCACAAGAGAGCCAAGGAAAGAGAGAAGAAGCTTCTGCGCAAAG
AACGAAGTCGGCTCAGAGTTCTCTCCGCCCCTGTTCTGTCCCAGCGTCTGCTTGGCATAT
CTGATGAACATGTGGAAGATCTATGCATGTCACTTAACACCGAGCAGTTGCGGAAACTGT
GTGACAAGATGGAAAACAAGGAAGGGATGGCTTTGGCAAAGGTGATCAAGAATGGGAGCA
ACATTGATGATGATAAAATAGAGATTGAAGAAGAAGAAGTTCAAGTAGCTGTTAAGCAGA
ATGGTCATATAGAAGCCAATGGTCATATAGAAGCCAATGGTCAT

Last Updated on 06.12.2018 16:11