Sequences from confirmation amplicon


Confirmation sequences for line 309B12 and hit At3g04730 are shown.
Line ID 309B12
Locus At3g04730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1289878
Primers used for PCR FV70 - 8474, show primer details
   
Sequence Name 28-K100895-0022-309-B12C-8409
GenBank Accession LN498759 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K100895-0022-309-B12C-8409
CATTTACAATTGTAGTTGTCCAAAGTTTATTTAATCTTTAATCTTTTTGGTAGATAAAGA
AAGATTCCTTATGTTTTGTATTTTTCTAGTTCTTCCCGACAAAAAAATAATCAAACAGAC
TCTCAACCTAACTACAATTGAAACATGGAGCAAAACCAAATAAAAAACAAAGATCTTAGG
AGAAGTATCAGCATAATTATTCCGTTCGAGTTCCCTAACCTAGCTAGAAATCATGTTAAA
GCGTATACATCTCTACATAATGACCGCCTAGTATAACCTAATTTTACCGAACCTAGAAGA
CAATATTATTAGTTGAAATAGAGATTGAAATTCAGTTTCTTACTTGGCTGGTGGTTTTAC
GACCTTCTCCTTCATATTCTCTAAATCGACTTTGGAAACTGAATCCATAGCCGTCGATGA
AAGATTCAGTTTGAGATCAACAGTCTCAGAAAATCCTCTTTTACCATTATTTTTTCCAGC
CATTTCTCCTCCGTGATTCCCACCCGGTAGCCCTAATCTCAGCTCCGTGGCCT
   
Sequence Name 28-K100903-0022-309-B12C-FV70
GenBank Accession LN498760 [GenBank]
Sequencing primer gene specific primer, FV70: 5'-AATATGATTAATTTTGAGGCCACG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K100903-0022-309-B12C-FV70
GATCCGGAGGAGAATGGCTGGAAAAATAATGGTAAAAGAGGATTTTCTGAGACTGTTGAT
CTCAAACTGAATCTTTCATCGACGGCTATGGATTCAGTTTCCAAAGTCGATTTAGAGAAT
ATGAAGGAGAAGGTCGTAAAACCACCAGCCAAGTAAGAAACTGAATTTCAATCTCTATTT
CAACTAATAATATTGTCTTCTAGGTTCGGTAAAATTAGGTTATACTAGGCGGTCATTATG
TAGAGATGTATACGCTTTAACATGATTTCTAGCTAGGTTAGGGAACTCGAACGGAATAAT
TATGCTGATACTTCTCCTAAGATCTTTGTTTTTTATTTGGTTTTGCTCCATGTTTCAATT
GTAGTTAGGTTGAGAGTCTGTTTGATTATTTTTTTGTCGGGAAGAACTAGAAAAATACAA
AACATAAGGAATCTTTCTTTATCTACCAAAAAGATTAAAGATTAAATAAACTTTTGGACA
ACTACNATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGT

Last Updated on 06.12.2018 16:11