Sequences from confirmation amplicon


Confirmation sequences for line 309H09 and hit At3g55810 are shown.
Line ID 309H09
Locus At3g55810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20712638
Primers used for PCR FV69 - 8474, show primer details
   
Sequence Name 30-K100895-0022-309-H09C-8409
GenBank Accession LN499637 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K100895-0022-309-H09C-8409
GCCTTTACATTGAATATATCCTGAACAAAATCGACATCATCGCTCTTTCTTTTGTTCGTA
AAGGATCTGACCTAACTGAAGTTAGGAAGTTACTTGGAGAACACTCAAAGAACATCATGC
TCATGTCAAAGGTGGAGAATCAAGAAGGGGTCATGAATTGTGAAAAGATTCTGGAGAATT
CTGATGCTTTCATGGTGGCTAGAGGAGATCTTGGAATGGAGATTCAGATCGAAAAGATGT
TCCTTGCTCAGAAAACAATGATCAAGATGGCTAATGCTCTTGGGAAACCAGTTGTCACAG
CCACACAGATGCTTGAATCCATGACAGTATCTCCTCGTCCGACTAGAGCTGAAGCCACTG
ACGTTGCAAACGCTGTTCTTGACGGGACAGATTGCGTTATGCTTAGTGGAGAAACCGCTG
CAGGAGCTCACCCTGAGGCTGCCGTGCTAACAATGTCAAGGATCTGTAAAGAAGCAGAGG
ATT
   
Sequence Name 30-K100903-0022-309-H09C-FV69
GenBank Accession LN499638 [GenBank]
Sequencing primer gene specific primer, FV69: 5'-GTAATCGATGAAATCCTCTGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K100903-0022-309-H09C-FV69
CACGGCAGCCTCAGGGTGAGCTCCTGCAGCGGTTTCTCCACTAAGCATAACGCAATCTGT
CCCGTCAAGAACAGCGTTTGCAACGTCAGTGGCTTCAGCTCTAGTCGGACGAGGAGATAC
TGTCATGGATTCAAGCATCTGTGTGGCTGTGACAACTGGTTTCCCAAGAGCATTAGCCAT
CTTGATCATTGTTTTCTGAGCAAGGAACATCTTTTCGATCTGAATCTCCATTCCAAGATC
TCCTCTAGCCACCATGAAAGCATCAGAATTCTCCAGAATCTTTTCACAATTCATGACCCC
TTCTTGATTCTCCACCTTTGACATGAGCATGATGTTCTTTGAGTGTTCTCCAAGTAACTT
CCTAACTTCAGTTAGGTCAGATCCTTTACGAACAAAAGAAAGAGCGATGATGTCGATTTT
GTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11