Sequences from confirmation amplicon


Confirmation sequences for line 313F08 and hit At5g16120 are shown.
Line ID 313F08
Locus At5g16120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5266918
Primers used for PCR 20vo - 8474, show primer details
   
Sequence Name 68-K117914-0022-313-F08C-20vo
GenBank Accession LN512090 [GenBank]
Sequencing primer gene specific primer, 20vo: 5'-TTGTTTTTCAATGTCCTGAGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K117914-0022-313-F08C-20vo
ACAATCATGTTGTATGGTGTCTGCATATATTAAACAGGTGGCTTAAGTAAGGAAATCGCA
AGAAGAAAAGGCGATACGTTCCTCAAAATCTGCTTTGTTTGTTTTTACCATATCCCTCTT
CCTGATATCTCTAAACCCTGCTTCTGCTAAATCCTTTTGTGGAACCAACTTGTGTTTTGG
TAGAACATTAGCTAGACCGATCAAAATCTGTTTCAATACAGGTGGTGGAACCAAATCATC
TGCAATCTAGAACGAATAGATATCTCATGTTAATGTTATGATTAGTAATCCCAGAGATTA
ATAACAAGGAAAAACAAGTCTTTACTTTGCACATTGGTGCTAACAAGACTGCGCCGGCCC
ACGCATTTGGTTGTTTGAGATGTATTTTGAGTGACACAGCTCCACCCATTGACTGTCCAA
AGAGAAAGCTCGGTAGAGAACTAAACTCAGGATTCGCTGCACGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAACATTA
TA
   
Sequence Name 28-K117913-0022-313-F08C-8409
GenBank Accession LN499028 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117913-0022-313-F08C-8409
TCATTGAATATATCCTGCAGCGAATCCTGAGTTTAGTTCTCTACCGAGCTTTCTCTTTGG
ACAGTCAATGGGTGGAGCTGTGTCACTCAAAATACATCTCAAACAACCAAATGCGTGGGC
CGGCGCAGTCTTGTTAGCACCAATGTGCAAAGTAAAGACTTGTTTTTCCTTGTTATTAAT
CTCTGGGATTACTAATCATAACATTAACATGAGATATCTATTCGTTCTAGATTGCAGATG
ATTTGGTTCCACCACCTGTATTGAAACAGATTTTGATCGGTCTAGCTAATGTTCTACCAA
AACACAAGTTGGTTCCACAAAAGGATTTAGCAGAAGCAGGGTTTAGAGATATCAGGAAGA
GGGATATGGTAAAAACAAACGAAGCAGATTTTGAGGAACGTATCGCCTTTTCTTCTTGCG
ATTTCCTTACTTAAGCCACCTGTTTAATATATGCAGACACCATACAACATGATTTGTTAC
AGCGGCAAACCACGGCTACGGACTGCAGTAAAAATGCTTCGAACAACTCAAGACATTGAA
AAAC

Last Updated on 06.12.2018 16:11