Sequences from confirmation amplicon


Confirmation sequences for line 314F02 and hit At1g12310 are shown.
Line ID 314F02
Locus At1g12310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4187716
Primers used for PCR IW42 - 8474, show primer details
   
Sequence Name 60-K033214-022-314-F02C-IW42
GenBank Accession LN510175 [GenBank]
Sequencing primer gene specific primer, IW42: 5'-AAGGCATAAAAGACAAACCTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K033214-022-314-F02C-IW42
ATCACCAGACAACATTTCCATTAAAAATGAAAACAAACATGAAGAAGACAAAGTTAGTAA
GCAAATTCCACAATGATAAAACAAACCCTAGCTAGGATTCGCTCGAAATCAAACAAACAA
GTGAAAAAAACACAAAGAATCTCTTCTTAAAAATTTCAACTTTCAAAACATAAAAGATTA
GATCTCACTTAGCAACCATCCTTGCTATGAAATCTTCATACCGGATCTTTCCATCGGATC
CAACATCCACCTCCTTGATCCACTCATCGAACTCATTAGGCTCCAGCTTCTCTCCGATAC
TGGTCAGAATATGCCTCAGATCCGCCACAGCAACGAACCCGGTACCTTCCTTATCGAGCA
CTTTGAATGCGTCACGGAGCTGGCGATCGAAAGGTTCCGTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 52-K033214-022-314-F02C-8409
GenBank Accession LN507724 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K033214-022-314-F02C-8409
GCCATTTACATTGACGGAACCTTTCGATCGCCAGCTCCGTGACGCATTCAAAGTGCTCGA
TAAGGAAGGTACCGGGTTCGTTGCTGTGGCGGATCTGAGGCATATTCTGACCAGTATCGG
AGAGAAGCTGGAGCCTAATGAGTTCGATGAGTGGATCAAGGAGGTGGATGTTGGATCCGA
TGGAAAGATCCGGTATGAAGATTTCATAGCAAGGATGGTTGCTAAGTGAGATCTAATCTT
TTATGTTTTGAAAGTTGAAATTTTTAAGAAGAGATTCTTTGTGTTTTTTTCACTTGTTTG
TTTGATTTCGAGCGAATCCTAGCTAGGGTTTGTTTTATCATTGTGGAATTTGCTTACTAA
CTTTGTCTTCTTCATGTTTGTTTTCATTTTTAATGGAAATGTTGTCTGTGTGATTCCTCT
TATCTTATCTAATCTCTAAGAATGTCAAGGTTTGTCTTTTATGCC

Last Updated on 06.12.2018 16:11