Sequences from confirmation amplicon


Confirmation sequences for line 314F08 and hit At1g51560 are shown.
Line ID 314F08
Locus At1g51560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19120408
Primers used for PCR 42ra - 8474, show primer details
   
Sequence Name 18-K115950-0022-314-F08C-42ra
GenBank Accession LN494450 [GenBank]
Sequencing primer gene specific primer, 42ra: 5'-CACTCGGAATATCTTAGCTGAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115950-0022-314-F08C-42ra
TCATCGTGCTTAACTATACAACATATTGGTGTTTACTCCCTTTTTTGTTCTCTGTGAATT
TGGTATTTTCAGATACCAGGATGGAGTTGCTTATCGAATGCAAGAGTAACATTATTCGGC
GATGTCTACCCATTGCCAGAAGAGCAGCAAGTGAGTGTCAATATGGAACAACACAGAATG
AGTAATACATGACCTGTTCAACACTGTTATTCCTGTTTCTAGTTCATTGGTTCTCTTGTT
CTGGATTTCTAGGAATGGGCCCATAAGCAGTATATGTTGAAGCATCACCAAGGACCTTCC
CAACAATGGGGAAACTTTCACTATTTTAGAATGCAGAACATAAGGTTTTTGTGTTCTTCT
TGAATTTCAGCCATTCCATAGTGTACTTATCAGAAAATAATACCAAGCTTGATTTTTTCT
CAGTGATATATATTTCATTGGAGGTTTTGGCACTGTCGCGTGGATCAATGTCAATGAGTA
CGAGACTCTTCAGCCAGATAAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGACCGCACGGTT
   
Sequence Name 08-K115949-0022-314-F08C-8409
GenBank Accession LN489878 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115949-0022-314-F08C-8409
TCCCGCGACAGTGCCAAAACCTCCAATGAAATATATATCACTGAGAAAAAATCAAGCTTG
GTATTATTTTCTGATAAGTACACTATGGAATGGCTGAAATTCAAGAAGAACACAAAAACC
TTATGTTCTGCATTCTAAAATAGTGAAAGTTTCCCCATTGTTGGGAAGGTCCTTGGTGAT
GCTTCAACATATACTGCTTATGGGCCCATTCCTAGAAATCCAGAACAAGAGAACCAATGA
ACTAGAAACAGGAATAACAGTGTTGAACAGGTCATGTATTACTCATTCTGTGTTGTTCCA
TATTGACACTCACTTGCTGCTCTTCTGGCAATGGGTAGACATCGCCGAATAATGTTACTC
TTGCATTCGATAAGCAACTCCATCCTGGTATCTGAAAATACCAAATTCACAGAGAACAAA
AAAGGGAGTAAACACCAATATGTTTGTATAGTTTAAGCACGATTGATAATGTTGTACCTG
AACAACGAGGGTGCATCTAGGTTCAGCTAAGAATTTTCCGAGTG

Last Updated on 06.12.2018 16:11